Characterization of Peripheral Neurovascular Capillary Structure
Location
CSU Ballroom
Start Date
21-4-2008 1:00 PM
End Date
21-4-2008 3:00 PM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Michael Bentley
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
Neurovasculature is critical to the peripheral nerve survival. Neurovascular development is well characterized, but little is known about the architecture of the microvasculature and capillary beds of peripheral nerves. Peripheral nerves from the lower extremities were attained from Wistar Kyoto rats and fixed by glutaraldehyde infusion into the vasculature. The vasculature was subsequently filled with PU4ii polyurethane-based resin to create a three-dimensional cast of the vasculature. The nerves were dissected after the resin completely polymerized. The nerves were placed in concentrated potassium hydroxide and progressively monitored via light microscopy. The nerves were stained with osmium, critical point dried, sputter coated with gold palladium and analyzed via scanning electron microscopy. Partially digested and completely digested specimens were analyzed. The methodology is relatively novel for examining high resolution lower extremity neurovasculature of the mammal model and may provide information related to regenerative medicine and reconstructive surgery.
Characterization of Peripheral Neurovascular Capillary Structure
CSU Ballroom
Neurovasculature is critical to the peripheral nerve survival. Neurovascular development is well characterized, but little is known about the architecture of the microvasculature and capillary beds of peripheral nerves. Peripheral nerves from the lower extremities were attained from Wistar Kyoto rats and fixed by glutaraldehyde infusion into the vasculature. The vasculature was subsequently filled with PU4ii polyurethane-based resin to create a three-dimensional cast of the vasculature. The nerves were dissected after the resin completely polymerized. The nerves were placed in concentrated potassium hydroxide and progressively monitored via light microscopy. The nerves were stained with osmium, critical point dried, sputter coated with gold palladium and analyzed via scanning electron microscopy. Partially digested and completely digested specimens were analyzed. The methodology is relatively novel for examining high resolution lower extremity neurovasculature of the mammal model and may provide information related to regenerative medicine and reconstructive surgery.
Recommended Citation
Sudbeck, Adam W. and Michael W. Witthaus. "Characterization of Peripheral Neurovascular Capillary Structure." Undergraduate Research Symposium, Mankato, MN, April 21, 2008.
https://cornerstone.lib.mnsu.edu/urs/2008/poster-session-B/22
