Extraction and Identification of Compounds in Bitter Leaf (Vernonia Amygdalina)
Location
CSU Ballroom
Start Date
21-4-2008 1:00 PM
End Date
21-4-2008 3:00 PM
Student's Major
Chemistry and Geology
Student's College
Science, Engineering and Technology
Mentor's Name
Danae Quirk Dorr
Mentor's Department
Chemistry and Geology
Mentor's College
Science, Engineering and Technology
Description
The focus of this proposal was to isolate and determine the chemical composition of Vernonia amygdalina (VA) in both blanched and unblanched leaves. Chemical extractions were accomplished with four different solvents: methanol, hexanes, ethyl acetate and water. Two sets of extractions were performed. In the first set of extractions, the leaves were ground and allowed to stir in each of these solvents. Filtration was then used to remove extract from the solid remnant. In the second set, a sohxlet extractor was used with the same solvents to further extract compounds from the solid remnant of the first extraction set. Thin layer chromatography (TLC) was used to separate the chemical constituents of the extraction. UV was used to indentify organic compounds containing conjugated double bonds. Analysis of UV active compounds after TLC indicated that VA contains at least four methanol soluble compounds, one hexane soluble compound, six ethyl acetate soluble compounds and three water soluble compounds. No compound was detected in the aqueous extraction of blanched VA. Further analysis of extractions using LCMS is currently being conducted.
Extraction and Identification of Compounds in Bitter Leaf (Vernonia Amygdalina)
CSU Ballroom
The focus of this proposal was to isolate and determine the chemical composition of Vernonia amygdalina (VA) in both blanched and unblanched leaves. Chemical extractions were accomplished with four different solvents: methanol, hexanes, ethyl acetate and water. Two sets of extractions were performed. In the first set of extractions, the leaves were ground and allowed to stir in each of these solvents. Filtration was then used to remove extract from the solid remnant. In the second set, a sohxlet extractor was used with the same solvents to further extract compounds from the solid remnant of the first extraction set. Thin layer chromatography (TLC) was used to separate the chemical constituents of the extraction. UV was used to indentify organic compounds containing conjugated double bonds. Analysis of UV active compounds after TLC indicated that VA contains at least four methanol soluble compounds, one hexane soluble compound, six ethyl acetate soluble compounds and three water soluble compounds. No compound was detected in the aqueous extraction of blanched VA. Further analysis of extractions using LCMS is currently being conducted.
Recommended Citation
Fru, Leonard Che. "Extraction and Identification of Compounds in Bitter Leaf (Vernonia Amygdalina)." Undergraduate Research Symposium, Mankato, MN, April 21, 2008.
https://cornerstone.lib.mnsu.edu/urs/2008/poster-session-B/23
