Discovering the Structural Interactions Between Potential Anti-Cancer Agent NSC 652287 and Guanine
Location
CSU Ballroom
Start Date
21-4-2008 1:00 PM
End Date
21-4-2008 3:00 PM
Student's Major
Chemistry and Geology
Student's College
Science, Engineering and Technology
Mentor's Name
Danae Quirk Dorr
Mentor's Department
Chemistry and Geology
Mentor's College
Science, Engineering and Technology
Description
The focus of this research was to standardize the cross-links that form between guanines when DNA is exposed to 2, 5-bis (5-hydroxymethyl-2-thienyl) furan, NSC 652287. NSC 652287 is derived from a family of thiophenes that are found in plants and have widely been used in medicine for therapeutic uses such as antibiotics, antifungal and antiviral compounds. Many of the details about how NSC 652287 reacts with DNA are unknown. NSC 652287 was examined for its activity toward guanosine and deoxyguanosine. Reactions with calf-thymus DNA and sequence specific oligonucleotides were also explored. The methods used in this research were derived from previous work done on similar alkylating compounds including crotonaldehyde and diepoxybutane. All samples were analyzed using liquid chromatography-mass spectrometry (LC/MS), and think layer chromatography (TLC). Analysis of LC/MS has shown that NSC 652287 reacts with the calf-thymus DNA, and TLC has shown that NSC 652287 reacts with guanine.
Discovering the Structural Interactions Between Potential Anti-Cancer Agent NSC 652287 and Guanine
CSU Ballroom
The focus of this research was to standardize the cross-links that form between guanines when DNA is exposed to 2, 5-bis (5-hydroxymethyl-2-thienyl) furan, NSC 652287. NSC 652287 is derived from a family of thiophenes that are found in plants and have widely been used in medicine for therapeutic uses such as antibiotics, antifungal and antiviral compounds. Many of the details about how NSC 652287 reacts with DNA are unknown. NSC 652287 was examined for its activity toward guanosine and deoxyguanosine. Reactions with calf-thymus DNA and sequence specific oligonucleotides were also explored. The methods used in this research were derived from previous work done on similar alkylating compounds including crotonaldehyde and diepoxybutane. All samples were analyzed using liquid chromatography-mass spectrometry (LC/MS), and think layer chromatography (TLC). Analysis of LC/MS has shown that NSC 652287 reacts with the calf-thymus DNA, and TLC has shown that NSC 652287 reacts with guanine.
Recommended Citation
Thompson, Mark. "Discovering the Structural Interactions Between Potential Anti-Cancer Agent NSC 652287 and Guanine." Undergraduate Research Symposium, Mankato, MN, April 21, 2008.
https://cornerstone.lib.mnsu.edu/urs/2008/poster-session-B/32
