Expression of 9/13 Hydroperoxide Lyase in Cucumber Leaves
Location
CSU 202
Start Date
27-4-2009 10:00 AM
End Date
27-4-2009 12:00 PM
Student's Major
Chemistry and Geology
Student's College
Science, Engineering and Technology
Mentor's Name
James Rife
Mentor's Department
Chemistry and Geology
Mentor's College
Science, Engineering and Technology
Description
Fatty Acid 9/13-Hydroperoxide Lyase (9/13-HPL) in the cucumber plant is an enzyme that cleaves either 9- or 13-hydroperoxides of polyunsaturated fatty acids to form volatile C9 or C6 aldehydes respectively. Since these aldehydes may play a role in the plant's defenses against pathogens (K. Matsui, et. al. Phytochemistry 67 (2006) 649-657), the enzyme is stress induced during vulnerable times such as when the plant is injured. In order to better understand how this enzyme is induced, we tested the effect of various factors on transcription of the 9/13-HPL gene. We specifically tested factors that have been shown to induce defense responses in other plant systems. Real Time Polymerase Chain Reaction was used to quantitate levels of 9/13-HPL mRNA. In initial experiments, the effect of mechanical wounding of cucumber cotyledons on the transcription levels of the 9/13-HPL gene in wounded tissue and unwounded leaves was examined. In subsequent experiments, the effects of mechanical wounding coupled with treatment with methyl jasmonate, ethylene or norborandiene (an ethylene antagonist), was tested. The RNeasy Plant Minikit from Qiagen was used to isolate mRNA from the plant tissue. SYBR Green was used as the detection system for Real Time PGR.
Expression of 9/13 Hydroperoxide Lyase in Cucumber Leaves
CSU 202
Fatty Acid 9/13-Hydroperoxide Lyase (9/13-HPL) in the cucumber plant is an enzyme that cleaves either 9- or 13-hydroperoxides of polyunsaturated fatty acids to form volatile C9 or C6 aldehydes respectively. Since these aldehydes may play a role in the plant's defenses against pathogens (K. Matsui, et. al. Phytochemistry 67 (2006) 649-657), the enzyme is stress induced during vulnerable times such as when the plant is injured. In order to better understand how this enzyme is induced, we tested the effect of various factors on transcription of the 9/13-HPL gene. We specifically tested factors that have been shown to induce defense responses in other plant systems. Real Time Polymerase Chain Reaction was used to quantitate levels of 9/13-HPL mRNA. In initial experiments, the effect of mechanical wounding of cucumber cotyledons on the transcription levels of the 9/13-HPL gene in wounded tissue and unwounded leaves was examined. In subsequent experiments, the effects of mechanical wounding coupled with treatment with methyl jasmonate, ethylene or norborandiene (an ethylene antagonist), was tested. The RNeasy Plant Minikit from Qiagen was used to isolate mRNA from the plant tissue. SYBR Green was used as the detection system for Real Time PGR.
Recommended Citation
Ranginwala, Samee. "Expression of 9/13 Hydroperoxide Lyase in Cucumber Leaves." Undergraduate Research Symposium, Mankato, MN, April 27, 2009.
https://cornerstone.lib.mnsu.edu/urs/2009/oral-session-05/2
