Determination of the Minimum Concentrations of Ampicillin Needed to Inhibit the Growth of Gram- Negative Bacteria Recovered from Tributaries of the Minnesota River
Location
CSU Ballroom
Start Date
28-4-2009 10:00 AM
End Date
28-4-2009 12:00 PM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Timothy E. Secott
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
There is a growing concern about antibiotics leaching from septic systems or from livestock operations into water systems, as this may contribute to the development of antimicrobial resistance in pathogenic organisms. Previous work has shown that organisms resistant to tetracycline and ampicillin are present in water from tributaries of the MN River. However, these results were performed using disk diffusion testing, which allows only qualitative assessment of susceptibility or resistance to antibiotics. The purpose of this study was to use minimum inhibitory concentration (MIC) testing to provide quantitative reevaluation of previous work, with an interest in detecting emerging trends in microbial resistance to ampicillin. We have demonstrated that our technique provides accurate reproducible results with Escherichia coli ATCC 25922, a quality control strain. MIC testing will be conducted on gram-negative organisms previously tested by disk diffusion. A comparison of these results will be presented, along with emerging general trends in ampicillin resistance.
Determination of the Minimum Concentrations of Ampicillin Needed to Inhibit the Growth of Gram- Negative Bacteria Recovered from Tributaries of the Minnesota River
CSU Ballroom
There is a growing concern about antibiotics leaching from septic systems or from livestock operations into water systems, as this may contribute to the development of antimicrobial resistance in pathogenic organisms. Previous work has shown that organisms resistant to tetracycline and ampicillin are present in water from tributaries of the MN River. However, these results were performed using disk diffusion testing, which allows only qualitative assessment of susceptibility or resistance to antibiotics. The purpose of this study was to use minimum inhibitory concentration (MIC) testing to provide quantitative reevaluation of previous work, with an interest in detecting emerging trends in microbial resistance to ampicillin. We have demonstrated that our technique provides accurate reproducible results with Escherichia coli ATCC 25922, a quality control strain. MIC testing will be conducted on gram-negative organisms previously tested by disk diffusion. A comparison of these results will be presented, along with emerging general trends in ampicillin resistance.
Recommended Citation
Abdi, Elias K.. "Determination of the Minimum Concentrations of Ampicillin Needed to Inhibit the Growth of Gram- Negative Bacteria Recovered from Tributaries of the Minnesota River." Undergraduate Research Symposium, Mankato, MN, April 28, 2009.
https://cornerstone.lib.mnsu.edu/urs/2009/poster-session-C/14
