Determination of the Minimum Concentrations of Ampicillin Needed to Inhibit the Growth of Gram- Negative Bacteria Recovered from Tributaries of the Minnesota River

Location

CSU Ballroom

Start Date

28-4-2009 10:00 AM

End Date

28-4-2009 12:00 PM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Timothy E. Secott

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

There is a growing concern about antibiotics leaching from septic systems or from livestock operations into water systems, as this may contribute to the development of antimicrobial resistance in pathogenic organisms. Previous work has shown that organisms resistant to tetracycline and ampicillin are present in water from tributaries of the MN River. However, these results were performed using disk diffusion testing, which allows only qualitative assessment of susceptibility or resistance to antibiotics. The purpose of this study was to use minimum inhibitory concentration (MIC) testing to provide quantitative reevaluation of previous work, with an interest in detecting emerging trends in microbial resistance to ampicillin. We have demonstrated that our technique provides accurate reproducible results with Escherichia coli ATCC 25922, a quality control strain. MIC testing will be conducted on gram-negative organisms previously tested by disk diffusion. A comparison of these results will be presented, along with emerging general trends in ampicillin resistance.

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Apr 28th, 10:00 AM Apr 28th, 12:00 PM

Determination of the Minimum Concentrations of Ampicillin Needed to Inhibit the Growth of Gram- Negative Bacteria Recovered from Tributaries of the Minnesota River

CSU Ballroom

There is a growing concern about antibiotics leaching from septic systems or from livestock operations into water systems, as this may contribute to the development of antimicrobial resistance in pathogenic organisms. Previous work has shown that organisms resistant to tetracycline and ampicillin are present in water from tributaries of the MN River. However, these results were performed using disk diffusion testing, which allows only qualitative assessment of susceptibility or resistance to antibiotics. The purpose of this study was to use minimum inhibitory concentration (MIC) testing to provide quantitative reevaluation of previous work, with an interest in detecting emerging trends in microbial resistance to ampicillin. We have demonstrated that our technique provides accurate reproducible results with Escherichia coli ATCC 25922, a quality control strain. MIC testing will be conducted on gram-negative organisms previously tested by disk diffusion. A comparison of these results will be presented, along with emerging general trends in ampicillin resistance.

Recommended Citation

Abdi, Elias K.. "Determination of the Minimum Concentrations of Ampicillin Needed to Inhibit the Growth of Gram- Negative Bacteria Recovered from Tributaries of the Minnesota River." Undergraduate Research Symposium, Mankato, MN, April 28, 2009.
https://cornerstone.lib.mnsu.edu/urs/2009/poster-session-C/14