Confirmation of the Polyglutamine Protein KIAA1946's Intracellular Localization.
Location
CSU Ballroom
Start Date
28-4-2009 10:00 AM
End Date
28-4-2009 12:00 PM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Geoffrey M. Goellner
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
There are approximately 20,000 genes within the human genome, and of these genes- over 40% are considered novel (genes of unknown function). As all genes code for proteins involved in important cellular processes, deciphering the normal function of unknown genes is essential to our overall understanding of how cells work, and may allow researchers to better identify gene abnormalities that affect human health. With the use of standard molecular/cell biological techniques (such as restriction digestion and electrophoresis), we have initiated studies to determine the cellular function of one such novel gene product called KIAA1946. As a first step, we have attempted to clone KIAA1946 into a vector containing a "FLAG" epitope- in order to create a FLAG-KIAA1946 fusion protein that we can use to localize our novel gene product in tissue culture cells. Localization in cells provides a good first clue regarding the function of a novel protein- such as KIAA1946. Our cloning strategy was to use restriction enzymes (Sail and BamHI) to cut KIAA1946 out of a pre-existing vector and splice it into the same restriction sites of our FLAG-vector. Once successfully cloned, we will perform immunolocalization experiments to compare FLAG-KIAA1946 location in cells relative to previous data showing GFP-KIAA1946 in cytoplasmic vesicles that are reminiscent of endosomes.
Confirmation of the Polyglutamine Protein KIAA1946's Intracellular Localization.
CSU Ballroom
There are approximately 20,000 genes within the human genome, and of these genes- over 40% are considered novel (genes of unknown function). As all genes code for proteins involved in important cellular processes, deciphering the normal function of unknown genes is essential to our overall understanding of how cells work, and may allow researchers to better identify gene abnormalities that affect human health. With the use of standard molecular/cell biological techniques (such as restriction digestion and electrophoresis), we have initiated studies to determine the cellular function of one such novel gene product called KIAA1946. As a first step, we have attempted to clone KIAA1946 into a vector containing a "FLAG" epitope- in order to create a FLAG-KIAA1946 fusion protein that we can use to localize our novel gene product in tissue culture cells. Localization in cells provides a good first clue regarding the function of a novel protein- such as KIAA1946. Our cloning strategy was to use restriction enzymes (Sail and BamHI) to cut KIAA1946 out of a pre-existing vector and splice it into the same restriction sites of our FLAG-vector. Once successfully cloned, we will perform immunolocalization experiments to compare FLAG-KIAA1946 location in cells relative to previous data showing GFP-KIAA1946 in cytoplasmic vesicles that are reminiscent of endosomes.
Recommended Citation
Erosmosele, Esther and Megan Fischer. "Confirmation of the Polyglutamine Protein KIAA1946's Intracellular Localization.." Undergraduate Research Symposium, Mankato, MN, April 28, 2009.
https://cornerstone.lib.mnsu.edu/urs/2009/poster-session-C/2
