Vasculature Regeneration Following Partial Hepatectomy in the Rat
Location
CSU Ballroom
Start Date
28-4-2009 10:00 AM
End Date
28-4-2009 12:00 PM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Michael Bentley
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
It is well known that when a portion of liver tissue is removed, the remaining tissue will undergo regeneration. Although much is known about the regeneration process, very little is known about the regrowth of the vasculature in the liver. To further analyze the regrowth of the vasculature, partial hepatectomies were performed on rats. Ten adult rats were used for this study. Five rats were used for sham surgeries and the other five were used for the partial hepatectomy. Two weeks after the surgeries, each rat was reopened and the vasculature of its liver was infused with 0.9% saline and 2.5% glutaraldehyde in 0.10 M phosphate buffer (pH 7.2). The livers were then post-fixed for one hour in 1% osmium tetroxide. The livers were dehydrated in acetone, critical point dried and studied by scanning electron microscopy. Two weeks after the hepatectomy, the remaining tissue of the experimental livers increased in size and had a mass comparable to the control. Vascular regrowth occurred in remaining lobes and not at the site of resection. The results of this study indicate that vascular regrowth is an integral part of liver regeneration and that new scar tissue that formed at the site of resection interfered with the regeneration process.
Vasculature Regeneration Following Partial Hepatectomy in the Rat
CSU Ballroom
It is well known that when a portion of liver tissue is removed, the remaining tissue will undergo regeneration. Although much is known about the regeneration process, very little is known about the regrowth of the vasculature in the liver. To further analyze the regrowth of the vasculature, partial hepatectomies were performed on rats. Ten adult rats were used for this study. Five rats were used for sham surgeries and the other five were used for the partial hepatectomy. Two weeks after the surgeries, each rat was reopened and the vasculature of its liver was infused with 0.9% saline and 2.5% glutaraldehyde in 0.10 M phosphate buffer (pH 7.2). The livers were then post-fixed for one hour in 1% osmium tetroxide. The livers were dehydrated in acetone, critical point dried and studied by scanning electron microscopy. Two weeks after the hepatectomy, the remaining tissue of the experimental livers increased in size and had a mass comparable to the control. Vascular regrowth occurred in remaining lobes and not at the site of resection. The results of this study indicate that vascular regrowth is an integral part of liver regeneration and that new scar tissue that formed at the site of resection interfered with the regeneration process.
Recommended Citation
Frank, Brittany and Sarah Karalus. "Vasculature Regeneration Following Partial Hepatectomy in the Rat." Undergraduate Research Symposium, Mankato, MN, April 28, 2009.
https://cornerstone.lib.mnsu.edu/urs/2009/poster-session-C/6
