Anatomical Characterization of the Rat Peripheral Neurovasculature
Location
CSU Ballroom
Start Date
28-4-2009 10:00 AM
End Date
28-4-2009 12:00 PM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Michael Bentley
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
Vascularization is essential in the continued existence and maintenance of the nervous system. Neurons are functional cells of the nervous system; they rely on a constant supply of oxygen and glucose supplied by capillaries. Information regarding the anatomical distribution of capillary beds amongst peripheral nerves is vital in the comprehension of the allocation of nutrients. Studies carried out previously demonstrated that the arterial supply and venous drainage were visible running parallel to the sciatic nerve of the Wistar Kyoto rat. Also, capillary networks were evident in the outer tissue layer with capillary structures extending into the deeper tissue. In continuance with these previous findings, the purpose of this study was to further characterize the morphology of the microvasculature of these peripheral. This was accomplished by observing the anatomical layout of the capillary beds and analyzing their interactions with the tissue of the peripheral nerves. Corrosion casting was used to examine the three-dimensional relationship between capillary beds and the proximal peripheral nerves. PU4ii resin was infused into the descending aorta of anesthetized Wistar Kyoto rats. The plastic was allowed to polymerize and the sciatic nerve was removed. In addition, sciatic nerves without infusion were removed for digestion. The sciatic nerves were placed in 0.25% collagenase and incubated for 1.25 hours at 3TC. After the partial enzymatic digestion, the nerves were fixed in 3% glutaraldehyde and prepared for scanning electron microscopy. The images obtained show the arrangement of the vasculature with the nerve fibers.
Anatomical Characterization of the Rat Peripheral Neurovasculature
CSU Ballroom
Vascularization is essential in the continued existence and maintenance of the nervous system. Neurons are functional cells of the nervous system; they rely on a constant supply of oxygen and glucose supplied by capillaries. Information regarding the anatomical distribution of capillary beds amongst peripheral nerves is vital in the comprehension of the allocation of nutrients. Studies carried out previously demonstrated that the arterial supply and venous drainage were visible running parallel to the sciatic nerve of the Wistar Kyoto rat. Also, capillary networks were evident in the outer tissue layer with capillary structures extending into the deeper tissue. In continuance with these previous findings, the purpose of this study was to further characterize the morphology of the microvasculature of these peripheral. This was accomplished by observing the anatomical layout of the capillary beds and analyzing their interactions with the tissue of the peripheral nerves. Corrosion casting was used to examine the three-dimensional relationship between capillary beds and the proximal peripheral nerves. PU4ii resin was infused into the descending aorta of anesthetized Wistar Kyoto rats. The plastic was allowed to polymerize and the sciatic nerve was removed. In addition, sciatic nerves without infusion were removed for digestion. The sciatic nerves were placed in 0.25% collagenase and incubated for 1.25 hours at 3TC. After the partial enzymatic digestion, the nerves were fixed in 3% glutaraldehyde and prepared for scanning electron microscopy. The images obtained show the arrangement of the vasculature with the nerve fibers.
Recommended Citation
Vreeman, Chelsea and Stacy Singfiel. "Anatomical Characterization of the Rat Peripheral Neurovasculature." Undergraduate Research Symposium, Mankato, MN, April 28, 2009.
https://cornerstone.lib.mnsu.edu/urs/2009/poster-session-C/7
