Effect of Lowered Aldosterone Levels on the Expression of the GR, MR, NHE-1, and NHE-3 in Rat Tissues
Location
CSU 253/4/5
Start Date
5-4-2010 10:00 AM
End Date
5-4-2010 12:00 PM
Student's Major
Chemistry and Geology
Student's College
Science, Engineering and Technology
Mentor's Name
Theresa Salerno
Mentor's Department
Chemistry and Geology
Mentor's College
Science, Engineering and Technology
Description
Hypertension is a major health concern among adults because it can lead to premature death due to cardiac arrest. Prenatal programming of adult hypertension has also been studied, but the causes are not fully understood. While there are various possible causes to this, aldosterone is a key hormone that binds to mineralocorticoid receptors (MR) and this elicits the production of protein responsible for blood volume and pressure. Because glucocortiocoid hormones can bind to both glucocorticoid receptors (GR) and MR, excess glucocorticoids have also been shown to contribute to hypertension. Additionally, studies have shown that sodium transport by the Na+/H+ exchanger (NHE) can participate in a hypertensive response (Pinto et al., 2008). In this study, outer layer of adrenal glands of rats were cryo-destructed to lower aldosterone levels.
The objective of this research was to look at the effect of lowered aldosterone levels on the expression of GR and NHE isoforms 1 and 3 in the kidneys and placentae tissues of spontaneously hypertensive rats (SHR) and non-hypertensive rats. RNA was isolated using RNeasy Plus Mini Kit (Qiagen) and quantified spectrophotometrically. Total RNAs were converted back to cDNA using reverse transcriptase and random primers. Primers and probes were designed for Quantitative PCR (qPCR) and have successfully been used to amplify target mRNAs. Preliminary results show no definitive differences between treated and untreated rat tissue but do show differences among rats.
Effect of Lowered Aldosterone Levels on the Expression of the GR, MR, NHE-1, and NHE-3 in Rat Tissues
CSU 253/4/5
Hypertension is a major health concern among adults because it can lead to premature death due to cardiac arrest. Prenatal programming of adult hypertension has also been studied, but the causes are not fully understood. While there are various possible causes to this, aldosterone is a key hormone that binds to mineralocorticoid receptors (MR) and this elicits the production of protein responsible for blood volume and pressure. Because glucocortiocoid hormones can bind to both glucocorticoid receptors (GR) and MR, excess glucocorticoids have also been shown to contribute to hypertension. Additionally, studies have shown that sodium transport by the Na+/H+ exchanger (NHE) can participate in a hypertensive response (Pinto et al., 2008). In this study, outer layer of adrenal glands of rats were cryo-destructed to lower aldosterone levels.
The objective of this research was to look at the effect of lowered aldosterone levels on the expression of GR and NHE isoforms 1 and 3 in the kidneys and placentae tissues of spontaneously hypertensive rats (SHR) and non-hypertensive rats. RNA was isolated using RNeasy Plus Mini Kit (Qiagen) and quantified spectrophotometrically. Total RNAs were converted back to cDNA using reverse transcriptase and random primers. Primers and probes were designed for Quantitative PCR (qPCR) and have successfully been used to amplify target mRNAs. Preliminary results show no definitive differences between treated and untreated rat tissue but do show differences among rats.
Recommended Citation
Kim, Ami. "Effect of Lowered Aldosterone Levels on the Expression of the GR, MR, NHE-1, and NHE-3 in Rat Tissues." Undergraduate Research Symposium, Mankato, MN, April 5, 2010.
https://cornerstone.lib.mnsu.edu/urs/2010/poster-session-A/7
