Determination of Strontium Incorporation in Bone
Location
CSU 253/4/5
Start Date
4-4-2011 11:00 AM
End Date
4-4-2011 12:30 PM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Michael Bentley
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
Strontium is a bone-seeking, trace element that has been shown through previous experiments to be a
―valuable safe alternative‖ for calcium absorption tests. To determine the replacement of calcium with strontium in C57/B16 mice, mice were given distilled water with 0.15 g strontium chloride per 100 mL for different periods of time. Some of the mice were also given a low calcium chow to reduce the amount of calcium available to them through their environment. Control mice were given distilled water and normal chow, or 0.105 g calcium chloride per 100 mL drinking water with normal or low calcium chow. After being on the specific diets for 3 weeks or longer, the mice were euthanized by CO2 inhalation and dissected to examine their femurs. The long bones were removed and stored in 2.5% glutaraldehyde, buffered with 0.1 M phosphate (pH 7.2) until they were ready for analysis by Energy Dispersive X-ray Spectroscopy (EDS) to determine elemental content of strontium. Early results have shown the presence of strontium in mice tibias. Further analysis of the various diets will lead to a better understanding of the effects of strontium chloride water with or without a low calcium diet.
Determination of Strontium Incorporation in Bone
CSU 253/4/5
Strontium is a bone-seeking, trace element that has been shown through previous experiments to be a
―valuable safe alternative‖ for calcium absorption tests. To determine the replacement of calcium with strontium in C57/B16 mice, mice were given distilled water with 0.15 g strontium chloride per 100 mL for different periods of time. Some of the mice were also given a low calcium chow to reduce the amount of calcium available to them through their environment. Control mice were given distilled water and normal chow, or 0.105 g calcium chloride per 100 mL drinking water with normal or low calcium chow. After being on the specific diets for 3 weeks or longer, the mice were euthanized by CO2 inhalation and dissected to examine their femurs. The long bones were removed and stored in 2.5% glutaraldehyde, buffered with 0.1 M phosphate (pH 7.2) until they were ready for analysis by Energy Dispersive X-ray Spectroscopy (EDS) to determine elemental content of strontium. Early results have shown the presence of strontium in mice tibias. Further analysis of the various diets will lead to a better understanding of the effects of strontium chloride water with or without a low calcium diet.
Recommended Citation
Fischer, Cassandra and Danielle Kral. "Determination of Strontium Incorporation in Bone." Undergraduate Research Symposium, Mankato, MN, April 4, 2011.
https://cornerstone.lib.mnsu.edu/urs/2011/poster-session-B/2
