Effects of Strontium in the Bone Density of Mice
Location
CSU Ballroom
Start Date
16-4-2013 10:00 AM
End Date
16-4-2013 12:00 PM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Michael Bentley
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
Dietary strontium is readily incorporated into bone tissue of rodents. In prior studies dietary strontium has been shown to inhibit calcium metabolism and has further been shown to prevent osteopenia in ovariectomized rats. In the present study, we evaluate changes in bone density of mice receiving low calcium diet and strontium chloride in the drinking water. The study includes 14 of male mice. A surgical procedure was performed to remove the testes from all of the mice through two small incisions in the scrotal area. The mice were put under anesthesia using isoflurane gas and received 0.02 ml dosage of Rimadyl post-surgery to help with pain. The surgery followed our Institutional Animal Care and Use Committee approval guidelines. All of the mice are weighed by group on a weekly basis to document weight gain and consumption of water and chow. After two months of the water and diet regimen the mice will be euthanized by carbon dioxide inhalation and the long bones will be dissected for analysis with a JEOL 6510 scanning electron microscope (SEM) equipped with a Thermo Noran silicon-drift energy dispersive spectroscopy (EDS) system. This system provides a means to measure strontium, calcium, phosphorous, and other mineral elements in bone tissue. We anticipate finding strontium incorporation and increased bone density in the mice using the Strontium Chloride and low calcium water and food regiment.
Effects of Strontium in the Bone Density of Mice
CSU Ballroom
Dietary strontium is readily incorporated into bone tissue of rodents. In prior studies dietary strontium has been shown to inhibit calcium metabolism and has further been shown to prevent osteopenia in ovariectomized rats. In the present study, we evaluate changes in bone density of mice receiving low calcium diet and strontium chloride in the drinking water. The study includes 14 of male mice. A surgical procedure was performed to remove the testes from all of the mice through two small incisions in the scrotal area. The mice were put under anesthesia using isoflurane gas and received 0.02 ml dosage of Rimadyl post-surgery to help with pain. The surgery followed our Institutional Animal Care and Use Committee approval guidelines. All of the mice are weighed by group on a weekly basis to document weight gain and consumption of water and chow. After two months of the water and diet regimen the mice will be euthanized by carbon dioxide inhalation and the long bones will be dissected for analysis with a JEOL 6510 scanning electron microscope (SEM) equipped with a Thermo Noran silicon-drift energy dispersive spectroscopy (EDS) system. This system provides a means to measure strontium, calcium, phosphorous, and other mineral elements in bone tissue. We anticipate finding strontium incorporation and increased bone density in the mice using the Strontium Chloride and low calcium water and food regiment.
Recommended Citation
Trukki, Kali; Ashley Ledding; and Breanna Ganther. "Effects of Strontium in the Bone Density of Mice." Undergraduate Research Symposium, Mankato, MN, April 16, 2013.
https://cornerstone.lib.mnsu.edu/urs/2013/poster-session-A/1
