The Study of the Effects of P-Anisaldehyde and 3,4-Dimethoxybenzaldehyde on 2'-Deoxyadenosine and 2'-Deoxyguanosine
Location
CSU Ballroom
Start Date
16-4-2013 10:00 AM
End Date
16-4-2013 12:00 PM
Student's Major
Chemistry and Geology
Student's College
Science, Engineering and Technology
Mentor's Name
Danae Quirk Dorr
Mentor's Department
Chemistry and Geology
Mentor's College
Science, Engineering and Technology
Description
Normal cells in the human body grow, divide, and then die very uniformly. If the normal cell’s DNA is damaged, the cell will either repair it or die. Aldehydes have been shown to induce the cell’s ability to repair damaged DNA. The analysis of p-anisaldehyde and 3,4-dimethoxybenzaldehyde’s ability to induce this repair is the goal of this research. The focus of this project was to synthesize possible products of these aldehydes with 2’-deoxyguanosine and 2’-deoxyadenosine. p- Anisaldehyde was allowed to react independently with 2’-deoxyguanosine and 2’-deoxyadenosine in dimethyl sulfoxide at 50OC for 24 hours. 3,4-Dimethoxybenzaldehyde was also allowed to react independently with 2’-deoxyguanosine and 2’-deoxyadenosine under the same conditions. Following the initial reaction 1H NMR spectra were analyzed and the reactions were repeated at 70OC for 48 hours. In addition, a DNA dissolved in Tris buffer was also allowed to be treated with each aldehyde at 40OC for 48 hours. After acid hydrolysis, all reaction mixtures were analyzed by HPLC.
The Study of the Effects of P-Anisaldehyde and 3,4-Dimethoxybenzaldehyde on 2'-Deoxyadenosine and 2'-Deoxyguanosine
CSU Ballroom
Normal cells in the human body grow, divide, and then die very uniformly. If the normal cell’s DNA is damaged, the cell will either repair it or die. Aldehydes have been shown to induce the cell’s ability to repair damaged DNA. The analysis of p-anisaldehyde and 3,4-dimethoxybenzaldehyde’s ability to induce this repair is the goal of this research. The focus of this project was to synthesize possible products of these aldehydes with 2’-deoxyguanosine and 2’-deoxyadenosine. p- Anisaldehyde was allowed to react independently with 2’-deoxyguanosine and 2’-deoxyadenosine in dimethyl sulfoxide at 50OC for 24 hours. 3,4-Dimethoxybenzaldehyde was also allowed to react independently with 2’-deoxyguanosine and 2’-deoxyadenosine under the same conditions. Following the initial reaction 1H NMR spectra were analyzed and the reactions were repeated at 70OC for 48 hours. In addition, a DNA dissolved in Tris buffer was also allowed to be treated with each aldehyde at 40OC for 48 hours. After acid hydrolysis, all reaction mixtures were analyzed by HPLC.
Recommended Citation
Bowman, Jenna. "The Study of the Effects of P-Anisaldehyde and 3,4-Dimethoxybenzaldehyde on 2'-Deoxyadenosine and 2'-Deoxyguanosine." Undergraduate Research Symposium, Mankato, MN, April 16, 2013.
https://cornerstone.lib.mnsu.edu/urs/2013/poster-session-A/38
