The Toxic Effects on the Liver and Kidney of Administering an Analgesic (Acetaminophen) and a Drug to Treat Obesity-Related Diabetes (Metformin) to Dietary-Induced Obese Male Mice (C57BL/6J)
Location
CSU Ballroom
Start Date
21-4-2014 10:00 AM
End Date
21-4-2014 11:30 AM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Steven Mercurio
Mentor's Email Address
steven.mercurio@mnsu.edu
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
Acetaminophen is an OTC pain reliever that causes liver damage on overdose. Lower doses affect the kidneys, especially in male mice were the liver metabolite is toxic. Metformin is a medication that is used for dietary-induced diabetes and is similarly toxic to the liver and kidney, especially if compromised by other damage already. Lactic acidosis can further damage organs. The effects of childhood obesity on this medication have not been fully evaluated. The model of dietary-induced obese male mouse is comparable. The expected outcome of a high dose of acetaminophen with metformin will be liver and kidney damage as seen in the organ and the blood chemistry changes. The null hypothesis is that these changes are not observed. The mice will have free access to 11% fat diet to further fatten them over a four week period, then will be put into four groups of four and begin their experimental treatments for four weeks. The four trials will be no medication, acetaminophen, metformin, and a mixture of acetaminophen and metformin. Elevated levels of bilirubin or creatinine may indicate liver or kidney problems and can monitor changes in organ function. Mice will be euthanized using CO2. Serum samples will be taken for a HPLC assay, as well as blood and tissue samples. Blood bilirubin levels will be measured using direct spectrophotometry and a tnf-alpha assay will be done using lysed cells. Kidneys and liver will be examined for physical damage, and tissue pH will be tested. Bodies disposed of by EHS.
The Toxic Effects on the Liver and Kidney of Administering an Analgesic (Acetaminophen) and a Drug to Treat Obesity-Related Diabetes (Metformin) to Dietary-Induced Obese Male Mice (C57BL/6J)
CSU Ballroom
Acetaminophen is an OTC pain reliever that causes liver damage on overdose. Lower doses affect the kidneys, especially in male mice were the liver metabolite is toxic. Metformin is a medication that is used for dietary-induced diabetes and is similarly toxic to the liver and kidney, especially if compromised by other damage already. Lactic acidosis can further damage organs. The effects of childhood obesity on this medication have not been fully evaluated. The model of dietary-induced obese male mouse is comparable. The expected outcome of a high dose of acetaminophen with metformin will be liver and kidney damage as seen in the organ and the blood chemistry changes. The null hypothesis is that these changes are not observed. The mice will have free access to 11% fat diet to further fatten them over a four week period, then will be put into four groups of four and begin their experimental treatments for four weeks. The four trials will be no medication, acetaminophen, metformin, and a mixture of acetaminophen and metformin. Elevated levels of bilirubin or creatinine may indicate liver or kidney problems and can monitor changes in organ function. Mice will be euthanized using CO2. Serum samples will be taken for a HPLC assay, as well as blood and tissue samples. Blood bilirubin levels will be measured using direct spectrophotometry and a tnf-alpha assay will be done using lysed cells. Kidneys and liver will be examined for physical damage, and tissue pH will be tested. Bodies disposed of by EHS.
Recommended Citation
Hofmann, Quinn; Yuko Nakamura; and Sara Sobota. "The Toxic Effects on the Liver and Kidney of Administering an Analgesic (Acetaminophen) and a Drug to Treat Obesity-Related Diabetes (Metformin) to Dietary-Induced Obese Male Mice (C57BL/6J)." Undergraduate Research Symposium, Mankato, MN, April 21, 2014.
https://cornerstone.lib.mnsu.edu/urs/2014/poster_session_A/15
