Location
CSU Ballroom
Start Date
21-4-2014 10:00 AM
End Date
21-4-2014 11:30 AM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Christopher Conlin
Mentor's Email Address
christopher.conlin@mnsu.edu
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
Salmonella enterica serovar Typhimurium, a bacterium frequently implicated in outbreaks of food poisoning, is able to survive in limiting phosphate environments by inducing a number of proteins that allow it to use a variety of compounds as phosphate sources. The gene apeE is induced when the cells are starved for phosphate. This gene encodes an outer membrane esterase that is not found in E. coli, and has been shown to be necessary for the growth of Salmonella Typhimurium in phosphate-limiting environments, such as those that may be encountered in an egg. To demonstrate the role of apeE in these environments, wild type and apeE mutant strains of Salmonella Typhimurium were separately tested for growth on minimal medium containing either phosphatidylcholine or lisophosphatidycholine as phosphate sources. Growth was observed for the wild type strain in the presence of both phosphate donors, but no growth was observed for the apeE mutant. To further support the role of apeE in the growth of Salmonella Typhimurium under phosphate-limiting conditions, other purified phospholipids as well as egg yolks will be tested for their ability to support the growth of both strains.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Included in
Role of the ApeE Esterase in the Growth of Salmonella on Phospholipids as Phosphate Sources
CSU Ballroom
Salmonella enterica serovar Typhimurium, a bacterium frequently implicated in outbreaks of food poisoning, is able to survive in limiting phosphate environments by inducing a number of proteins that allow it to use a variety of compounds as phosphate sources. The gene apeE is induced when the cells are starved for phosphate. This gene encodes an outer membrane esterase that is not found in E. coli, and has been shown to be necessary for the growth of Salmonella Typhimurium in phosphate-limiting environments, such as those that may be encountered in an egg. To demonstrate the role of apeE in these environments, wild type and apeE mutant strains of Salmonella Typhimurium were separately tested for growth on minimal medium containing either phosphatidylcholine or lisophosphatidycholine as phosphate sources. Growth was observed for the wild type strain in the presence of both phosphate donors, but no growth was observed for the apeE mutant. To further support the role of apeE in the growth of Salmonella Typhimurium under phosphate-limiting conditions, other purified phospholipids as well as egg yolks will be tested for their ability to support the growth of both strains.
Recommended Citation
Menuba, Stella. "Role of the ApeE Esterase in the Growth of Salmonella on Phospholipids as Phosphate Sources." Undergraduate Research Symposium, Mankato, MN, April 21, 2014.
https://cornerstone.lib.mnsu.edu/urs/2014/poster_session_A/24
