Analysis of Lipoxygenase 9 and 10 in Soybean Root by Q-PCR
Location
CSU Ballroom
Start Date
21-4-2014 10:00 AM
End Date
21-4-2014 11:30 AM
Student's College
Science, Engineering and Technology
Mentor's Name
James Rife
Mentor's Email Address
james.rife@mnsu.edu
Mentor's Department
Chemistry and Geology
Mentor's College
Science, Engineering and Technology
Description
Lipoxygenases are nonheme iron-containing enzymes which catalyze the addition of molecular oxygen to polyunsaturated fatty acids to form fatty acid hydroperoxides. Lipoxygenases are known to be involved in plant growth and development, lipid metabolism and nitrogen storage. Plants such as soybeans have many different lipoxygenase forms. LOX9 and LOX10 are two lipoxygenase forms that are less studied than others. It has been reported that the gene for LOX9 was highly induced in soybean roots infected with soybean cyst nematode, and LOX9 and LOX10 are involved in the development of nodules in soybean roots. Soybean plants (Edamame “BeSweet 292”) were grown for 6 weeks. The plants were separated into four groups. One group was untreated to be used as control; another group was not watered to see the effect of drought while the last two groups were treated with growth regulation hormones Abscisic Acid and Auxin. After the plants were separated and treated, the roots were harvested. RNA was isolated using a MicroRNA isolation kit from Applied Biosystems. The large mRNA fraction was used for this project. Once RNA was isolated, cDNA copies of the mRNAs were prepared using Reverse Transcription. Quantitative Polymerase Chain Reaction (Q-PCR) was used to measure changes in the levels of the cDNAs for LOX9 and LOX10. SYBR Green was used to monitor the Q-PCR reaction.
Analysis of Lipoxygenase 9 and 10 in Soybean Root by Q-PCR
CSU Ballroom
Lipoxygenases are nonheme iron-containing enzymes which catalyze the addition of molecular oxygen to polyunsaturated fatty acids to form fatty acid hydroperoxides. Lipoxygenases are known to be involved in plant growth and development, lipid metabolism and nitrogen storage. Plants such as soybeans have many different lipoxygenase forms. LOX9 and LOX10 are two lipoxygenase forms that are less studied than others. It has been reported that the gene for LOX9 was highly induced in soybean roots infected with soybean cyst nematode, and LOX9 and LOX10 are involved in the development of nodules in soybean roots. Soybean plants (Edamame “BeSweet 292”) were grown for 6 weeks. The plants were separated into four groups. One group was untreated to be used as control; another group was not watered to see the effect of drought while the last two groups were treated with growth regulation hormones Abscisic Acid and Auxin. After the plants were separated and treated, the roots were harvested. RNA was isolated using a MicroRNA isolation kit from Applied Biosystems. The large mRNA fraction was used for this project. Once RNA was isolated, cDNA copies of the mRNAs were prepared using Reverse Transcription. Quantitative Polymerase Chain Reaction (Q-PCR) was used to measure changes in the levels of the cDNAs for LOX9 and LOX10. SYBR Green was used to monitor the Q-PCR reaction.
Recommended Citation
Yahya, Robera. "Analysis of Lipoxygenase 9 and 10 in Soybean Root by Q-PCR." Undergraduate Research Symposium, Mankato, MN, April 21, 2014.
https://cornerstone.lib.mnsu.edu/urs/2014/poster_session_A/46
