Internalization of Escherichia coli (E. coli) into Lettuce Leaves through Stomata; Bruised vs. Unbruised leaves
Location
CSU Ballroom
Start Date
20-4-2015 10:00 AM
End Date
20-4-2015 11:30 AM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Michael Bentley
Mentor's Email Address
michael.bentley@mnsu.edu
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
The purpose of this experiment is to find out whether bruising or physical damage of lettuce leaves will significantly increase the internalization of E. coli through to the stomata. To investigate this we cut fresh lettuce leaves into four pairs of (6 mm x 6 mm) pieces and incubated each pair for 6 hours in four petri dishes. We bruised two pairs of specimen using forceps and left the other two pairs intact. One bruised pair and one unbruised pair of specimen were incubated in 1/10th concentration of E. coli, while a control group of one pair bruised and another pair unbruised were incubated in a solution with no E. coli. After incubation, we fixed the specimen in a fixing solution and prepared them for viewing under scanning electron microscope. Upon viewing the specimen under SEM, we have noted that more bacteria clusters were internalized in and around the stomata of bruised leaves incubated in E. coli than any of the other groups. As of now this result generally suggests that bruised leaves are more susceptible to E. coli internalization.
Internalization of Escherichia coli (E. coli) into Lettuce Leaves through Stomata; Bruised vs. Unbruised leaves
CSU Ballroom
The purpose of this experiment is to find out whether bruising or physical damage of lettuce leaves will significantly increase the internalization of E. coli through to the stomata. To investigate this we cut fresh lettuce leaves into four pairs of (6 mm x 6 mm) pieces and incubated each pair for 6 hours in four petri dishes. We bruised two pairs of specimen using forceps and left the other two pairs intact. One bruised pair and one unbruised pair of specimen were incubated in 1/10th concentration of E. coli, while a control group of one pair bruised and another pair unbruised were incubated in a solution with no E. coli. After incubation, we fixed the specimen in a fixing solution and prepared them for viewing under scanning electron microscope. Upon viewing the specimen under SEM, we have noted that more bacteria clusters were internalized in and around the stomata of bruised leaves incubated in E. coli than any of the other groups. As of now this result generally suggests that bruised leaves are more susceptible to E. coli internalization.
Recommended Citation
Limeneh, Jote and Lucy Saidu. "Internalization of Escherichia coli (E. coli) into Lettuce Leaves through Stomata; Bruised vs. Unbruised leaves." Undergraduate Research Symposium, Mankato, MN, April 20, 2015.
https://cornerstone.lib.mnsu.edu/urs/2015/poster_session_A/14
