The Role of MicroRNA 393 in Defense Against Phytophthora Sojae in Soybeans
Location
CSU Ballroom
Start Date
20-4-2015 10:00 AM
End Date
20-4-2015 11:30 AM
Student's Major
Chemistry and Geology
Student's College
Science, Engineering and Technology
Mentor's Name
James Rife
Mentor's Email Address
james.rife@mnsu.edu
Mentor's Department
Chemistry and Geology
Mentor's College
Science, Engineering and Technology
Description
Phytophthora sojae (P. sojae) is the second most destructive pathogen to soybeans; however, the mechanism of its pathogenesis is not well understood. Recent studies have shown that microRNA 393 (miR393) is up-regulated in response to P. sojae infection. Methyl jasmonate (MeJa) is produced by plants in response to wounding. To probe for a possible link between P. sojae infection and a response elicited by MeJa, soybean plants were treated to three conditions and then miR393 expression was monitored using Quantitative Polymerase Chain Reaction (Q-PCR). Treatment groups consisted of a control set of plants, P. sojae-infected plants, and plants treated with MeJa. Soybean roots were inoculated by scraping the soybean roots 1.0 cm from the root end and applying a small plug of P. sojae-infected agar. The MeJa group was treated by exposing the plants to evaporated MeJa in an enclosed container. 8 hours post-treatment, root tissues were harvested and miRNAs were isolated using an Ambion Plant RNA Isolation Aid in conjunction with a mirVana miRNA isolation kit. The quality and quantity of small RNAs was assessed by spectrophotometric analysis and by denaturing polyacrylamide electrophoresis. miRNAs were reverse transcribed into cDNA using a TaqMan microRNA Reverse Transcription System. Q-PCR, with FAM-labeled probes, and the ΔΔCt method were used to measure relative miR393 levels using U6 as an endogenous control.
The Role of MicroRNA 393 in Defense Against Phytophthora Sojae in Soybeans
CSU Ballroom
Phytophthora sojae (P. sojae) is the second most destructive pathogen to soybeans; however, the mechanism of its pathogenesis is not well understood. Recent studies have shown that microRNA 393 (miR393) is up-regulated in response to P. sojae infection. Methyl jasmonate (MeJa) is produced by plants in response to wounding. To probe for a possible link between P. sojae infection and a response elicited by MeJa, soybean plants were treated to three conditions and then miR393 expression was monitored using Quantitative Polymerase Chain Reaction (Q-PCR). Treatment groups consisted of a control set of plants, P. sojae-infected plants, and plants treated with MeJa. Soybean roots were inoculated by scraping the soybean roots 1.0 cm from the root end and applying a small plug of P. sojae-infected agar. The MeJa group was treated by exposing the plants to evaporated MeJa in an enclosed container. 8 hours post-treatment, root tissues were harvested and miRNAs were isolated using an Ambion Plant RNA Isolation Aid in conjunction with a mirVana miRNA isolation kit. The quality and quantity of small RNAs was assessed by spectrophotometric analysis and by denaturing polyacrylamide electrophoresis. miRNAs were reverse transcribed into cDNA using a TaqMan microRNA Reverse Transcription System. Q-PCR, with FAM-labeled probes, and the ΔΔCt method were used to measure relative miR393 levels using U6 as an endogenous control.
Recommended Citation
Oeltjenbruns, Andrew. "The Role of MicroRNA 393 in Defense Against Phytophthora Sojae in Soybeans." Undergraduate Research Symposium, Mankato, MN, April 20, 2015.
https://cornerstone.lib.mnsu.edu/urs/2015/poster_session_A/43
