Analysis of Sex Steroid Hormone Implants on Serum Levels of Two Steroid Hormones in the Green Anole Lizard
Location
CSU Ballroom
Start Date
20-4-2015 10:00 AM
End Date
20-4-2015 11:30 AM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Rachel Cohen
Mentor's Email Address
rachel.cohen@mnsu.edu
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
Sex steroid hormones and their derivatives have a broad effect on animal behavior and physiology, and treatment with testosterone and estradiol has been shown to be important for the production of new neurons in the adult brain. However, data shows that administration methods of steroid hormones are not always reliable, thus necessitating further investigation. We are testing the effects of implantation of various sex steroid hormones (testosterone and estradiol) in the green anole lizard (Anolis carolinensis). We collected serum from gonadectomized male lizards that were given a subcutaneous hormone pellet that contained testosterone, estradiol, or was left empty as a control. In order to assess delivery of sex steroid hormones, plasma testosterone and estradiol levels will be assayed via enzyme-linked immunosorbent assay (ELISA). We expect to find that we will be able to detect a difference in plasma hormone concentrations between testosterone- and estradiol-treated animals compared to control animals. This result would suggest that implantation of the hormonal pellet successfully increased serum levels in vivo.
Analysis of Sex Steroid Hormone Implants on Serum Levels of Two Steroid Hormones in the Green Anole Lizard
CSU Ballroom
Sex steroid hormones and their derivatives have a broad effect on animal behavior and physiology, and treatment with testosterone and estradiol has been shown to be important for the production of new neurons in the adult brain. However, data shows that administration methods of steroid hormones are not always reliable, thus necessitating further investigation. We are testing the effects of implantation of various sex steroid hormones (testosterone and estradiol) in the green anole lizard (Anolis carolinensis). We collected serum from gonadectomized male lizards that were given a subcutaneous hormone pellet that contained testosterone, estradiol, or was left empty as a control. In order to assess delivery of sex steroid hormones, plasma testosterone and estradiol levels will be assayed via enzyme-linked immunosorbent assay (ELISA). We expect to find that we will be able to detect a difference in plasma hormone concentrations between testosterone- and estradiol-treated animals compared to control animals. This result would suggest that implantation of the hormonal pellet successfully increased serum levels in vivo.
Recommended Citation
Connolly, Patrick. "Analysis of Sex Steroid Hormone Implants on Serum Levels of Two Steroid Hormones in the Green Anole Lizard." Undergraduate Research Symposium, Mankato, MN, April 20, 2015.
https://cornerstone.lib.mnsu.edu/urs/2015/poster_session_A/7
