Effect of Abiotic Stress on the Expression of Auxin Signaling F-Box 2 mRNA
Location
CSU Ballroom
Start Date
18-4-2016 10:00 AM
End Date
18-4-2016 11:30 AM
Student's Major
Chemistry and Geology
Student's College
Science, Engineering and Technology
Mentor's Name
James Rife
Mentor's Department
Chemistry and Geology
Mentor's College
Science, Engineering and Technology
Description
Auxin plays important roles in plant growth and development including stem elongation, phototropic and gravitropic responses, apical dominance, and lateral and adventitious root formation. A plant’s response to auxin involves Auxin/Indole-3-acetic acid (Aux/IAA) proteins, Auxin Response Factors (ARFs), Auxin Signaling F-Box Proteins (AFB1, AFB2 and AFB3) and components of the protein degradation pathway. Due to plants being immobile, environmental stress can be detrimental to their productivity. Plants have evolved defense mechanisms to cope with these environmental stressors. These defense mechanisms often impact the auxin signaling pathway. The goal of this project was to investigate the effect of environmental stress on expression of AFB-2, Auxin Signaling F-Box2. Four groups of Glycine max (soybean) plants were grown. While one group served as a control, the other groups were treated with abiotic stressors including drought, cold temperature, and high salinity. mRNAs were isolated using a mirVana miRNA Isolation kit (Life Technologies). cDNA copies of the isolated mRNAs were made using a High-Capacity cDNA Reverse Transcription kit (Applied Biosystems). Finally, Quantitative Polymerase Chain Reaction was performed to determine the relative quantities of the cDNA. The stresses had no significant effect on the expression of AFB2 in the leaves. However, root samples showed a 50% decrease in expression of AFB2 in drought and cold treated plants, whereas the high salinity treatment doubled the expression of AFB2 compared to the control.
Effect of Abiotic Stress on the Expression of Auxin Signaling F-Box 2 mRNA
CSU Ballroom
Auxin plays important roles in plant growth and development including stem elongation, phototropic and gravitropic responses, apical dominance, and lateral and adventitious root formation. A plant’s response to auxin involves Auxin/Indole-3-acetic acid (Aux/IAA) proteins, Auxin Response Factors (ARFs), Auxin Signaling F-Box Proteins (AFB1, AFB2 and AFB3) and components of the protein degradation pathway. Due to plants being immobile, environmental stress can be detrimental to their productivity. Plants have evolved defense mechanisms to cope with these environmental stressors. These defense mechanisms often impact the auxin signaling pathway. The goal of this project was to investigate the effect of environmental stress on expression of AFB-2, Auxin Signaling F-Box2. Four groups of Glycine max (soybean) plants were grown. While one group served as a control, the other groups were treated with abiotic stressors including drought, cold temperature, and high salinity. mRNAs were isolated using a mirVana miRNA Isolation kit (Life Technologies). cDNA copies of the isolated mRNAs were made using a High-Capacity cDNA Reverse Transcription kit (Applied Biosystems). Finally, Quantitative Polymerase Chain Reaction was performed to determine the relative quantities of the cDNA. The stresses had no significant effect on the expression of AFB2 in the leaves. However, root samples showed a 50% decrease in expression of AFB2 in drought and cold treated plants, whereas the high salinity treatment doubled the expression of AFB2 compared to the control.
Recommended Citation
Dusek, Dane. "Effect of Abiotic Stress on the Expression of Auxin Signaling F-Box 2 mRNA." Undergraduate Research Symposium, Mankato, MN, April 18, 2016.
https://cornerstone.lib.mnsu.edu/urs/2016/poster-session-A/35
