The Expression of Lipoxygenase Isoforms in Response to Wounding in Peas
Location
CSU Ballroom
Start Date
18-4-2016 10:00 AM
End Date
18-4-2016 11:30 AM
Student's Major
Chemistry and Geology
Student's College
Science, Engineering and Technology
Mentor's Name
Theresa Salerno
Mentor's Department
Chemistry and Geology
Mentor's College
Science, Engineering and Technology
Description
Peas are an important leguminous crop and, like soybeans, are subject to loss in yields because of infection and abiotic stresses. Lipoxygenases are enzymes that function in many roles in plant development and defense. They exist in multiple forms and have been implicated in the wound response. Veronico’s research group studied the expression of a specific type of LOX called LOXN2 in pea after mechanical wounding. Their data showed an increase in the expression of LOXN2 and this occurred three hours after wounding. Limited studies have been done in pea leaves. In this research study, Little Marvel pea seedlings were grown for 14 days. Then the leaves for one set of plants were wounded and the second set of plants were left untreated. Plants tissues were harvested 0, 4, 8, 12, and 24 hours after wounding and immediately frozen in liquid nitrogen. The messenger RNAs from the plant tissues were purified by using the mirVANA microRNA Isolation Kit. Quality and quantity of the larger RNAs were assessed using a Nanodrop Lite instrument. Total cDNAs were obtained using random hexamers and a High Capacity cDNA RT-PCR Kit. Primers and probes were designed to specifically measure the expression of LOXN2 and LOXN3 as well as elongation factor-1 alpha as the endogenous control. Initial qPCR results gave standard curves with high PCR efficiencies of 99.25% and 99.75% for the LOXN3 target and for EF1- alpha, respectively. It is hypothesized that the expression of LOXN2 and LOXN3 will increase after mechanical wounding.
The Expression of Lipoxygenase Isoforms in Response to Wounding in Peas
CSU Ballroom
Peas are an important leguminous crop and, like soybeans, are subject to loss in yields because of infection and abiotic stresses. Lipoxygenases are enzymes that function in many roles in plant development and defense. They exist in multiple forms and have been implicated in the wound response. Veronico’s research group studied the expression of a specific type of LOX called LOXN2 in pea after mechanical wounding. Their data showed an increase in the expression of LOXN2 and this occurred three hours after wounding. Limited studies have been done in pea leaves. In this research study, Little Marvel pea seedlings were grown for 14 days. Then the leaves for one set of plants were wounded and the second set of plants were left untreated. Plants tissues were harvested 0, 4, 8, 12, and 24 hours after wounding and immediately frozen in liquid nitrogen. The messenger RNAs from the plant tissues were purified by using the mirVANA microRNA Isolation Kit. Quality and quantity of the larger RNAs were assessed using a Nanodrop Lite instrument. Total cDNAs were obtained using random hexamers and a High Capacity cDNA RT-PCR Kit. Primers and probes were designed to specifically measure the expression of LOXN2 and LOXN3 as well as elongation factor-1 alpha as the endogenous control. Initial qPCR results gave standard curves with high PCR efficiencies of 99.25% and 99.75% for the LOXN3 target and for EF1- alpha, respectively. It is hypothesized that the expression of LOXN2 and LOXN3 will increase after mechanical wounding.
Recommended Citation
Ngumah, Iheme. "The Expression of Lipoxygenase Isoforms in Response to Wounding in Peas." Undergraduate Research Symposium, Mankato, MN, April 18, 2016.
https://cornerstone.lib.mnsu.edu/urs/2016/poster-session-A/36
