Transcriptome Analysis of Staph. Aureus in Response to Specific IgY through RNA-Sequencing
Location
CSU Ballroom
Start Date
11-4-2017 10:00 AM
End Date
11-4-2017 11:30 AM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Allison Land
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
Antibodies, also known as immunoglobulins (Ig), bind specific antigens, usually from bacteria or virus, and stimulate the immune system to respond accordingly. Camas Incorporated of Minnesota has produced patents for preventing and or decreasing respiratory illness in livestock. This is done by adding antigen-specific IgY to animal feed. They found that delivering the IgY via intranasal spray within feedlots resulted in the reduction of morbidity or mortality in certain agriculture animals. While treatments such as these are known to be effective, bacteria are notorious for their ability to evolve resistance. Therefore, I hypothesize that the binding of specific IgY to S. aureus will alter RNA transcription, resulting in the up regulating of pathogenic factors, compared to the control. Staphylococcus aureus was isolated from a field sample obtained by Camas Incorporated and will be used in this experiment due to its known detrimental effects to livestock. A positive control culture will contain an antibiotic, known to alter transcription, and addition of a non-binding IgY will serve as a negative control. The experimental condition will contain S. aureus and specific IgY. Antibodies will be purified from yolks of eggs which were laid by chickens immunized against S. aureus. RNA will then be purified from culture samples, reverse-transcribed into cDNA and prepared for DNA sequencing. Analysis of the transcriptome will be performed using various software. On examination of the differential transcriptional data, I anticipate being able to determine if S. aureus experienced altered regulation of pathogenic genes in response to IgY binding.
Transcriptome Analysis of Staph. Aureus in Response to Specific IgY through RNA-Sequencing
CSU Ballroom
Antibodies, also known as immunoglobulins (Ig), bind specific antigens, usually from bacteria or virus, and stimulate the immune system to respond accordingly. Camas Incorporated of Minnesota has produced patents for preventing and or decreasing respiratory illness in livestock. This is done by adding antigen-specific IgY to animal feed. They found that delivering the IgY via intranasal spray within feedlots resulted in the reduction of morbidity or mortality in certain agriculture animals. While treatments such as these are known to be effective, bacteria are notorious for their ability to evolve resistance. Therefore, I hypothesize that the binding of specific IgY to S. aureus will alter RNA transcription, resulting in the up regulating of pathogenic factors, compared to the control. Staphylococcus aureus was isolated from a field sample obtained by Camas Incorporated and will be used in this experiment due to its known detrimental effects to livestock. A positive control culture will contain an antibiotic, known to alter transcription, and addition of a non-binding IgY will serve as a negative control. The experimental condition will contain S. aureus and specific IgY. Antibodies will be purified from yolks of eggs which were laid by chickens immunized against S. aureus. RNA will then be purified from culture samples, reverse-transcribed into cDNA and prepared for DNA sequencing. Analysis of the transcriptome will be performed using various software. On examination of the differential transcriptional data, I anticipate being able to determine if S. aureus experienced altered regulation of pathogenic genes in response to IgY binding.
Recommended Citation
Woyda, Reed. "Transcriptome Analysis of Staph. Aureus in Response to Specific IgY through RNA-Sequencing." Undergraduate Research Symposium, Mankato, MN, April 11, 2017.
https://cornerstone.lib.mnsu.edu/urs/2017/poster-session-A/22
