Bromoxynil Octanoate Affect on Bacterium Acidovorax facilis
Location
CSU Ballroom
Start Date
10-4-2018 10:00 AM
End Date
10-4-2018 11:30 AM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Steven Mercurio
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
Bromoxynil is the second most applied herbicide (mass vs. acreage) will be tested for its effect on the soil nitrogen reducing bacteria, Acidovorax facilis. This is a free living aerobic soil bacterium. The study will consist of three test groups including a control receiving distilled water, an acetone and water mixture group and a group of acetone, water and bromoxynil treatment group. The EPA's limit on bromoxynil is 0.5 lbs. per acre which equates to 0.58 mg bromoxynil per 4 in. square glass vase. Each of the 3 experimental groups will have 3 replicates. Due to the low solubility of bromoxynil, it will be dissolved in 30 ul acetone (solubility 1.2 mg/ ul) and then added to 100 ml water and added to the soil samples. Bacteria will be cultured in ATCC medium 72 at 30°C for 2 days. Bacteria will be counted after incubation. To analyze change over time, 1g samples from all groups will be taken after bacteria are added to the soil, after liquid treatment has been added, two weeks after treatment and one month after treatment. The expected result is reduced plate count between pretreatment to one month after treatment comparing control and bromoxynil treatment groups. Results of the experiment will show if important nitrogen cycle bacteria are reduced in fitness and thus reduce soil quality in agricultural land. Bromoxynil is present in soil for about 60 days after application which would reduce soil health into the growing season.
Bromoxynil Octanoate Affect on Bacterium Acidovorax facilis
CSU Ballroom
Bromoxynil is the second most applied herbicide (mass vs. acreage) will be tested for its effect on the soil nitrogen reducing bacteria, Acidovorax facilis. This is a free living aerobic soil bacterium. The study will consist of three test groups including a control receiving distilled water, an acetone and water mixture group and a group of acetone, water and bromoxynil treatment group. The EPA's limit on bromoxynil is 0.5 lbs. per acre which equates to 0.58 mg bromoxynil per 4 in. square glass vase. Each of the 3 experimental groups will have 3 replicates. Due to the low solubility of bromoxynil, it will be dissolved in 30 ul acetone (solubility 1.2 mg/ ul) and then added to 100 ml water and added to the soil samples. Bacteria will be cultured in ATCC medium 72 at 30°C for 2 days. Bacteria will be counted after incubation. To analyze change over time, 1g samples from all groups will be taken after bacteria are added to the soil, after liquid treatment has been added, two weeks after treatment and one month after treatment. The expected result is reduced plate count between pretreatment to one month after treatment comparing control and bromoxynil treatment groups. Results of the experiment will show if important nitrogen cycle bacteria are reduced in fitness and thus reduce soil quality in agricultural land. Bromoxynil is present in soil for about 60 days after application which would reduce soil health into the growing season.
Recommended Citation
Olson, Erik. "Bromoxynil Octanoate Affect on Bacterium Acidovorax facilis." Undergraduate Research Symposium, Mankato, MN, April 10, 2018.
https://cornerstone.lib.mnsu.edu/urs/2018/poster-session-A/14
