Dissecting the Role of Serum Growth Hormone in the Regulation of Brain Igf-1
Location
CSU Ballroom
Start Date
10-4-2018 10:00 AM
End Date
10-4-2018 11:30 AM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
David Sharlin
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
Congenital hypothyroidism, a condition in which there is an insufficient amount of circulating thyroid hormone starting at birth, results in permanent neurological deficits. Interestingly, low Insulin-like growth factor 1 (Igf1) from birth results in similar neuroanatomical and cognitive deficits to that of congenital hypothyroidism. Furthermore, it has been demonstrated that thyroid hormone regulates pituitary growth hormone production and that pituitary-derived growth hormone regulates serum Igf1. However, it is unclear whether pituitary-derived growth hormone (GH) regulates local brain-derived Igf1. If reductions in brain Igf1 are related to deficits associated with low thyroid hormone, then recovering brain Igf1 may potentially help to normalize brain development. To test this idea, four groups of mice were used: euthyroid+saline, euthyroid+GH, hypothyroid+saline and hypothyroid+GH. Methimazole, a thyroid hormone inhibitor was delivered through water to pregnant dams starting on embryonic day14 (E14) to induce developmental hypothyroidism. Euthyroid and hypothyroid pups were injected with saline (vehicle) or GH from postnatal day 1 (P1) to P7. Pups were sacrificed on P7 and the brains and serum were collected. Preliminary results indicated that treatment had no effect on pup body or brain wet weights. The effect of treatment on serum GH concentrations will be measured using the enzyme-linked immunosorbent assay (ELISA). On-going experiments are quantifying brain Igf1 mRNA from total mRNA isolated by micro-dissection using SYBR-Green based quantitative real time-PCR. These findings will determine whether circulating levels of growth hormone regulate brain Igf1 mRNA levels, and whether thyroid hormone is an important factor in the expression of brain-derived Igf1.
Dissecting the Role of Serum Growth Hormone in the Regulation of Brain Igf-1
CSU Ballroom
Congenital hypothyroidism, a condition in which there is an insufficient amount of circulating thyroid hormone starting at birth, results in permanent neurological deficits. Interestingly, low Insulin-like growth factor 1 (Igf1) from birth results in similar neuroanatomical and cognitive deficits to that of congenital hypothyroidism. Furthermore, it has been demonstrated that thyroid hormone regulates pituitary growth hormone production and that pituitary-derived growth hormone regulates serum Igf1. However, it is unclear whether pituitary-derived growth hormone (GH) regulates local brain-derived Igf1. If reductions in brain Igf1 are related to deficits associated with low thyroid hormone, then recovering brain Igf1 may potentially help to normalize brain development. To test this idea, four groups of mice were used: euthyroid+saline, euthyroid+GH, hypothyroid+saline and hypothyroid+GH. Methimazole, a thyroid hormone inhibitor was delivered through water to pregnant dams starting on embryonic day14 (E14) to induce developmental hypothyroidism. Euthyroid and hypothyroid pups were injected with saline (vehicle) or GH from postnatal day 1 (P1) to P7. Pups were sacrificed on P7 and the brains and serum were collected. Preliminary results indicated that treatment had no effect on pup body or brain wet weights. The effect of treatment on serum GH concentrations will be measured using the enzyme-linked immunosorbent assay (ELISA). On-going experiments are quantifying brain Igf1 mRNA from total mRNA isolated by micro-dissection using SYBR-Green based quantitative real time-PCR. These findings will determine whether circulating levels of growth hormone regulate brain Igf1 mRNA levels, and whether thyroid hormone is an important factor in the expression of brain-derived Igf1.
Recommended Citation
Onadipe, Anjola and Madison Onadipe. "Dissecting the Role of Serum Growth Hormone in the Regulation of Brain Igf-1." Undergraduate Research Symposium, Mankato, MN, April 10, 2018.
https://cornerstone.lib.mnsu.edu/urs/2018/poster-session-A/15
