Antibiotic Resistant Escherichia coli (E. coli) in Minneopa Creek Collected During Two Storm Events in 2017
Location
CSU Ballroom
Start Date
10-4-2018 10:00 AM
End Date
10-4-2018 11:30 AM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Beth Proctor
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
State wide, the 3rd largest water impairment is E. coli/fecal coliform (approximately 600 listed with 83 added in 2016). E. coli is used to indicate the potential presence of waterborne pathogens that can be harmful to human health. Minnesota's water quality standard for E. coli in streams is 126 organisms per 100 mL (milliliters) of water. Potential sources of E. coli contamination include wildlife, failing septic systems, feedlots, wastewater treatment plant effluents, and agricultural application of manure and treated biosolids to fields. E. coli levels were monitored at several sites along Minneopa Creek during storms on June 28 and August 9, 2017 where rainfall was 0.68 and 1.56 inches, respectively. Nine of the 10 stream/ditch sites exceeded the standard and all but one of the sites were above the designated impaired water segment. E. coli were tested for resistance to 8 antibiotics (Amoxicillin/Clavulanic Acid, Cefotaxime, Ciprofloxacin, Colistin, Erythromycin, Neomycin, Oxytetracycline and Rifampin) using the Kirby-Bauer disk diffusion assay. In June, at Minneopa State Park (Park) 75% percent of the colonies tested were resistant to 4 or more antibiotics and all were developing resistance to 1 or more antibiotics and in August, at the Park 52% were resistant to 4 or more antibiotics and 83% were developing resistance to 1 or more antibiotics. E.coli were most resistant to Amoxicillin/Clavulanic Acid, Erythromycin, Neomycin, Oxytetracycline, Rifampin and Colistin. Resistance to Amoxicillin/Clavulanic Acid, Erythromycin and Colistin is especially concerning as these antibiotics are listed on the World Health Organization's list of essential medicines.
Antibiotic Resistant Escherichia coli (E. coli) in Minneopa Creek Collected During Two Storm Events in 2017
CSU Ballroom
State wide, the 3rd largest water impairment is E. coli/fecal coliform (approximately 600 listed with 83 added in 2016). E. coli is used to indicate the potential presence of waterborne pathogens that can be harmful to human health. Minnesota's water quality standard for E. coli in streams is 126 organisms per 100 mL (milliliters) of water. Potential sources of E. coli contamination include wildlife, failing septic systems, feedlots, wastewater treatment plant effluents, and agricultural application of manure and treated biosolids to fields. E. coli levels were monitored at several sites along Minneopa Creek during storms on June 28 and August 9, 2017 where rainfall was 0.68 and 1.56 inches, respectively. Nine of the 10 stream/ditch sites exceeded the standard and all but one of the sites were above the designated impaired water segment. E. coli were tested for resistance to 8 antibiotics (Amoxicillin/Clavulanic Acid, Cefotaxime, Ciprofloxacin, Colistin, Erythromycin, Neomycin, Oxytetracycline and Rifampin) using the Kirby-Bauer disk diffusion assay. In June, at Minneopa State Park (Park) 75% percent of the colonies tested were resistant to 4 or more antibiotics and all were developing resistance to 1 or more antibiotics and in August, at the Park 52% were resistant to 4 or more antibiotics and 83% were developing resistance to 1 or more antibiotics. E.coli were most resistant to Amoxicillin/Clavulanic Acid, Erythromycin, Neomycin, Oxytetracycline, Rifampin and Colistin. Resistance to Amoxicillin/Clavulanic Acid, Erythromycin and Colistin is especially concerning as these antibiotics are listed on the World Health Organization's list of essential medicines.
Recommended Citation
Jones, Melissa and Caitlin Flynn. "Antibiotic Resistant Escherichia coli (E. coli) in Minneopa Creek Collected During Two Storm Events in 2017." Undergraduate Research Symposium, Mankato, MN, April 10, 2018.
https://cornerstone.lib.mnsu.edu/urs/2018/poster-session-A/36
