Evaluating Adduct Formation Between 5'-Hydroxymethylfurfural (HMF) and DNA in the Presence of Arginine

Presenter Information

Diamond Yusuf, Minnesota State University, Mankato

Location

CSU Ballroom

Start Date

2-4-2019 10:00 AM

End Date

2-4-2019 11:30 AM

Student's Major

Chemistry and Geology

Student's College

Science, Engineering and Technology

Mentor's Name

Danae Quirk Dorr

Mentor's Department

Chemistry and Geology

Mentor's College

Science, Engineering and Technology

Description

5-Hydroxymethylfurfural (HMF) is a six carbon heterocyclic aldehyde that is formed through the Maillard reaction. Natural forms of this compound are found in carbohydrate rich sources, such as dried fruits and caramel products. HMF has been demonstrated to be toxic in animals with a link to its metabolite 5-sulfooxymethylfurfural (SMF). To date, little is known about the formation of SMF or the formation of DNA adducts in animals or humans. However, previous conclusions led to the central hypothesis of this project which is that DNA adducts will be formed in a reaction between DNA and HMF. Therefore, the main research goal was to determine if the formation of DNA adducts occurred. This was accomplished with several reactions containing variations of ct-DNA, 2'-deoxyguanosine, and L-arginine. The reactions were treated with either enzyme treatments - DNase and phosphodiesterase-or acid treatment–HCl, filtered, and then analyzed by high performance liquid chromatography (HPLC).