Exploring the Formation of Interstrand Crosslinks in Calf Thymus DNA with Arginine and Potential Anti-Cancer Agent SOS

Location

CSU Ballroom

Start Date

2-4-2019 10:00 AM

End Date

2-4-2019 11:30 AM

Student's Major

Chemistry and Geology

Student's College

Science, Engineering and Technology

Mentor's Name

Danae Quirk Dorr

Mentor's Department

Chemistry and Geology

Mentor's College

Science, Engineering and Technology

Description

One reason that cancer is a leading cause of death worldwide is its lack of termination programming. A possible solution to negating the effects of cancerous cells is to reprogram their DNA to replicate the termination process of non-cancerous cells. Forming interstrand crosslinks between the 2 helices of the DNA in cancer cells could halt the transcription of DNA. This would prevent the cancer cell from producing its necessary proteins and would eventually kill it. In this research, it has been proposed that reacting the thiophene derivative, SOS, in the presence of the amino acid arginine, with calf thymus DNA will form interstrand crosslinks in the DNA because SOS contains two aldehyde functional groups that can react with the guanosine nitrogenous base in DNA. In this experiment, the previously discussed reaction between SOS, arginine, and calf-thymus DNA was performed along with other couplings of the reactants used as controls so that results could be compared. Following the reaction, the calf thymus DNA mixtures underwent enzymatic digestion in order to cleave the nucleosides and nucleoside adducts from the phosphate sugar backbone. Each reaction sample underwent high-performance liquid chromatography (HPLC) for analysis.

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Apr 2nd, 10:00 AM Apr 2nd, 11:30 AM

Exploring the Formation of Interstrand Crosslinks in Calf Thymus DNA with Arginine and Potential Anti-Cancer Agent SOS

CSU Ballroom

One reason that cancer is a leading cause of death worldwide is its lack of termination programming. A possible solution to negating the effects of cancerous cells is to reprogram their DNA to replicate the termination process of non-cancerous cells. Forming interstrand crosslinks between the 2 helices of the DNA in cancer cells could halt the transcription of DNA. This would prevent the cancer cell from producing its necessary proteins and would eventually kill it. In this research, it has been proposed that reacting the thiophene derivative, SOS, in the presence of the amino acid arginine, with calf thymus DNA will form interstrand crosslinks in the DNA because SOS contains two aldehyde functional groups that can react with the guanosine nitrogenous base in DNA. In this experiment, the previously discussed reaction between SOS, arginine, and calf-thymus DNA was performed along with other couplings of the reactants used as controls so that results could be compared. Following the reaction, the calf thymus DNA mixtures underwent enzymatic digestion in order to cleave the nucleosides and nucleoside adducts from the phosphate sugar backbone. Each reaction sample underwent high-performance liquid chromatography (HPLC) for analysis.

Recommended Citation

Osgood, Abbigail. "Exploring the Formation of Interstrand Crosslinks in Calf Thymus DNA with Arginine and Potential Anti-Cancer Agent SOS." Undergraduate Research Symposium, Mankato, MN, April 2, 2019.
https://cornerstone.lib.mnsu.edu/urs/2019/poster-session-A/9