Formation of DNA Adducts with 2,5-Thiophenedicarboxaldehyde and Arginine

Presenter Information

Shelby Bonnemier, Minnesota State University, Mankato

Location

CSU Ballroom

Start Date

2-4-2019 2:00 PM

End Date

2-4-2019 3:30 PM

Student's Major

Chemistry and Geology

Student's College

Science, Engineering and Technology

Mentor's Name

Danae Quirk Dorr

Mentor's Department

Chemistry and Geology

Mentor's College

Science, Engineering and Technology

Description

Certain aldehydes, when in the presence of the amino acid arginine, can react with DNA and form DNA adducts. 2,5-Thiophenedicarboxaldehyde is a compound consisting of a five membered aromatic ring containing sulfur. Aldehydes with structures similar to 2,5-thiophenedicarboxaldehyde have been found to form DNA adducts and therefore have the ability to kill cancer cells. In this project, the necessary compounds 2,5-thiophenedicarboxaldehyde, arginine, calf-thymus DNA (ct-DNA), and deoxyguanosine were combined in equimolar amounts in buffer. After reacting, the enzymes DNase I, alkaline phosphatase, and phosphodiesterase I were used in order to prepare the samples for analysis. Following filtration, high-performance liquid chromatography (HPLC) was utilized as a way to qualitatively analyze the reaction products.