Effect Of Disinfectants On The Formation Of Biofilms By Methicillin-Resistant Staphylococcus aureus (MRSA)

Location

CSU Ballroom

Start Date

12-4-2022 2:00 PM

End Date

12-4-2022 3:30 PM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Lois Anderson

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

Methicillin-Resistant Staphylococcus aureus (MRSA) are Gram-positive cocci that cause a variety of bacterial infections. Infections involving biofilm-forming bacteria are extremely difficult to eradicate because biofilms impair antibiotic penetration and prevent normal immune responses. Biofilms are colonizations of bacteria in an extracellular polymeric substance matrix (EPS) that can attach to surfaces of an abiotic or biotic nature. The EPS made by the bacteria creates a barrier against antibiotics. To test the protectiveness of the biofilm created by Methicillin-Resistant Staphylococcus aureus, three strains were exposed to several disinfectants. The three strains of bacteria tested were 25923 (Methicillin-Sensitive Staphylococcus aureus (MSSA) forming no biofilms), 43300 (MRSA forming mediocre biofilms), and 6583 (MRSA forming strong biofilms). Biofilm formation assays were performed by using 24-hour bacterial cultures grown in TSB with 0.5% glucose at 370C. The cell suspensions were plated in a 96-well microplate and incubated for 24 hours at 370C in wells with no disinfectant and in wells pre-treated with different disinfectants. Uninoculated media was used as a background for the study. Wells were then washed with sterile distilled water. Cells were fixed with 99.9% methanol, stained with 1% crystal violet, rewashed with distilled water, and destained with 95% ethanol. A spectrophotometer analysis of optical density at 570 nm was used to determine the formation of a biofilm. The hypothesis is for the disinfectants to terminate the creation of a biofilm since disinfectants are used to kill or inhibit bacteria on several surfaces. Understanding the types of disinfectants that successfully halt the formation of staphylococcal biofilms can reduce the spread of infections caused by MRSA in hospitals or other environmental settings. The study will reveal the effects of different disinfectants on biofilm formation by Methicillin-Resistant Staphylococcus aureus.

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Apr 12th, 2:00 PM Apr 12th, 3:30 PM

Effect Of Disinfectants On The Formation Of Biofilms By Methicillin-Resistant Staphylococcus aureus (MRSA)

CSU Ballroom

Methicillin-Resistant Staphylococcus aureus (MRSA) are Gram-positive cocci that cause a variety of bacterial infections. Infections involving biofilm-forming bacteria are extremely difficult to eradicate because biofilms impair antibiotic penetration and prevent normal immune responses. Biofilms are colonizations of bacteria in an extracellular polymeric substance matrix (EPS) that can attach to surfaces of an abiotic or biotic nature. The EPS made by the bacteria creates a barrier against antibiotics. To test the protectiveness of the biofilm created by Methicillin-Resistant Staphylococcus aureus, three strains were exposed to several disinfectants. The three strains of bacteria tested were 25923 (Methicillin-Sensitive Staphylococcus aureus (MSSA) forming no biofilms), 43300 (MRSA forming mediocre biofilms), and 6583 (MRSA forming strong biofilms). Biofilm formation assays were performed by using 24-hour bacterial cultures grown in TSB with 0.5% glucose at 370C. The cell suspensions were plated in a 96-well microplate and incubated for 24 hours at 370C in wells with no disinfectant and in wells pre-treated with different disinfectants. Uninoculated media was used as a background for the study. Wells were then washed with sterile distilled water. Cells were fixed with 99.9% methanol, stained with 1% crystal violet, rewashed with distilled water, and destained with 95% ethanol. A spectrophotometer analysis of optical density at 570 nm was used to determine the formation of a biofilm. The hypothesis is for the disinfectants to terminate the creation of a biofilm since disinfectants are used to kill or inhibit bacteria on several surfaces. Understanding the types of disinfectants that successfully halt the formation of staphylococcal biofilms can reduce the spread of infections caused by MRSA in hospitals or other environmental settings. The study will reveal the effects of different disinfectants on biofilm formation by Methicillin-Resistant Staphylococcus aureus.

Recommended Citation

Turnipseed, Hannah. "Effect Of Disinfectants On The Formation Of Biofilms By Methicillin-Resistant Staphylococcus aureus (MRSA)." Undergraduate Research Symposium, Mankato, MN, April 12, 2022.
https://cornerstone.lib.mnsu.edu/urs/2022/poster-session-02/4