Abstract
Proteins containing polyglutamine (polyQ) tracts within their primary amino acid sequence are particularly interesting because expansion mutation within them has been shown to underlie a growing list of severe neurodegenerative disorders including Huntington's Disease and several types of Spinocerebellar Ataxias. FAM171B is a novel polyQ protein that was originally identified via large scale sequencing efforts. However, to date, very little is known regarding its normal cellular function and expression pattern. In this study, in situ hybridization was utilized to assess whether FAM171B is expressed in developing (postnatal days 7, 21, 42) and adult mouse brains. The results of the experiments performed suggest that FAM171B is indeed expressed in the brain, with pronounced expression in the hippocampus, Purkinje cells of the cerebellum, and cerebral cortex. Given these findings, FAM171B should be considered a candidate gene for exploration of molecularly uncharacterized neurodegenerative diseases.
Advisor
Geoffrey Goellner
Committee Member
David Sharlin
Committee Member
Rachel Cohen
Date of Degree
2017
Language
english
Document Type
Thesis
Degree
Master of Science (MS)
Department
Biological Sciences
College
Science, Engineering and Technology
Recommended Citation
Sudasinghe, A. K. (2017). Localization of FAM171B mRNA Expression In Mouse Brain Using In-Situ Hybridization [Master’s thesis, Minnesota State University, Mankato]. Cornerstone: A Collection of Scholarly and Creative Works for Minnesota State University, Mankato. https://cornerstone.lib.mnsu.edu/etds/735/
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