Expression of Total Fibronectin Protein in Spontaneously Hypertensive Rat Aorta and Atria as a Function of Age, Gender, and Exercise
Location
CSU
Student's Major
Chemistry and Geology
Student's College
Science, Engineering and Technology
Mentor's Name
Theresa Salerno
Mentor's Department
Chemistry and Geology
Mentor's College
Science, Engineering and Technology
Description
Fibronectin (FN) is a large glycoprotein dimer (500 kDa) that exists in either plasma or the extracellular matrix. The two monomer chains are similar (~250 kDa each) and are linked together by disulFide bonds. FN has multiple functions and is found in many different tissues and organs. Previous research has shown that there is a relationship between hypertension and age with respect to expression of FN in spontaneously hypertensive rats (SHR). This research studied the expression of total FN protein in SHR aorta and atria as a function of age, gender, and exercise. Protein was extracted from rat aorta and atria and total protein was measured. The Western blot technique was used to determine the amount of total FN present in each specific variable via chemiluminescence and densitometry. The difference in total FN protein will be discussed.
Expression of Total Fibronectin Protein in Spontaneously Hypertensive Rat Aorta and Atria as a Function of Age, Gender, and Exercise
CSU
Fibronectin (FN) is a large glycoprotein dimer (500 kDa) that exists in either plasma or the extracellular matrix. The two monomer chains are similar (~250 kDa each) and are linked together by disulFide bonds. FN has multiple functions and is found in many different tissues and organs. Previous research has shown that there is a relationship between hypertension and age with respect to expression of FN in spontaneously hypertensive rats (SHR). This research studied the expression of total FN protein in SHR aorta and atria as a function of age, gender, and exercise. Protein was extracted from rat aorta and atria and total protein was measured. The Western blot technique was used to determine the amount of total FN present in each specific variable via chemiluminescence and densitometry. The difference in total FN protein will be discussed.
