Expression of Fibronection Isoforms in Spontaneously Hypertensive Rat Aorta and Atria as a Function of Age, Gender, and Exercise
Location
CSU
Student's Major
Chemistry and Geology
Student's College
Science, Engineering and Technology
Mentor's Name
Theresa Salerno
Mentor's Department
Chemistry and Geology
Mentor's College
Science, Engineering and Technology
Description
Fibronectin (FN) is a large glycoprotein dimer (500 kDa) that exists in either plasma or the extracellular matrix. FN contains different protein isoforms (A, B, and V) with various molecular sizes. This is a result of alternative splicing of the FN pre-mRNA at three positions. Previous studies have shown the expression of different amounts of some FN isoforms as a function of aging and hypertension. This research project studied the expression of different FN isoforms in spontaneously hypertensive rat (SHR) aorta and atria as a function of age, gender, and exercise. RNA was isolated from each tissue for specific amplification in the reverse transcription polymerase chain reaction (RT-PCR) procedure. The different RNA isoforms for each variable were separated on gel electrophoresis and were quantitated by densitometry after silver staining. The differences in FN isoform expression will be discussed.
Expression of Fibronection Isoforms in Spontaneously Hypertensive Rat Aorta and Atria as a Function of Age, Gender, and Exercise
CSU
Fibronectin (FN) is a large glycoprotein dimer (500 kDa) that exists in either plasma or the extracellular matrix. FN contains different protein isoforms (A, B, and V) with various molecular sizes. This is a result of alternative splicing of the FN pre-mRNA at three positions. Previous studies have shown the expression of different amounts of some FN isoforms as a function of aging and hypertension. This research project studied the expression of different FN isoforms in spontaneously hypertensive rat (SHR) aorta and atria as a function of age, gender, and exercise. RNA was isolated from each tissue for specific amplification in the reverse transcription polymerase chain reaction (RT-PCR) procedure. The different RNA isoforms for each variable were separated on gel electrophoresis and were quantitated by densitometry after silver staining. The differences in FN isoform expression will be discussed.
