Expression of Fibronection Isoforms in Spontaneously Hypertensive Rat Aorta and Atria as a Function of Age, Gender, and Exercise

Location

CSU

Student's Major

Chemistry and Geology

Student's College

Science, Engineering and Technology

Mentor's Name

Theresa Salerno

Mentor's Department

Chemistry and Geology

Mentor's College

Science, Engineering and Technology

Description

Fibronectin (FN) is a large glycoprotein dimer (500 kDa) that exists in either plasma or the extracellular matrix. FN contains different protein isoforms (A, B, and V) with various molecular sizes. This is a result of alternative splicing of the FN pre-mRNA at three positions. Previous studies have shown the expression of different amounts of some FN isoforms as a function of aging and hypertension. This research project studied the expression of different FN isoforms in spontaneously hypertensive rat (SHR) aorta and atria as a function of age, gender, and exercise. RNA was isolated from each tissue for specific amplification in the reverse transcription polymerase chain reaction (RT-PCR) procedure. The different RNA isoforms for each variable were separated on gel electrophoresis and were quantitated by densitometry after silver staining. The differences in FN isoform expression will be discussed.

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Expression of Fibronection Isoforms in Spontaneously Hypertensive Rat Aorta and Atria as a Function of Age, Gender, and Exercise

CSU

Fibronectin (FN) is a large glycoprotein dimer (500 kDa) that exists in either plasma or the extracellular matrix. FN contains different protein isoforms (A, B, and V) with various molecular sizes. This is a result of alternative splicing of the FN pre-mRNA at three positions. Previous studies have shown the expression of different amounts of some FN isoforms as a function of aging and hypertension. This research project studied the expression of different FN isoforms in spontaneously hypertensive rat (SHR) aorta and atria as a function of age, gender, and exercise. RNA was isolated from each tissue for specific amplification in the reverse transcription polymerase chain reaction (RT-PCR) procedure. The different RNA isoforms for each variable were separated on gel electrophoresis and were quantitated by densitometry after silver staining. The differences in FN isoform expression will be discussed.