Identification of Interacting Proteins

Location

CSU

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Marilyn Hart

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

Capping protein (CP) is an actin binding protein that associates with the actin cytoskeleton and regulates its assembly. CP, found in all eucaryotic cells, is a heterodimer composed of α and β subunits. Three β subunits are found in CP (β1, β2, β3) and these receptors exist in all vertebrate organisms. In previous work, we have shown that the β1 and β2 subunits have distinct functions in cardiac myocytes that cannot be carried out by the other isoform. Furthermore, our data suggests that the unique function of the β isoforms Involves their interactions with an additional protein. We are using a genetic screen, a yeast two-hybrid screen, to identify proteins that interact with each β subunit.

This document is currently not available here.

Share

COinS
 

Identification of Interacting Proteins

CSU

Capping protein (CP) is an actin binding protein that associates with the actin cytoskeleton and regulates its assembly. CP, found in all eucaryotic cells, is a heterodimer composed of α and β subunits. Three β subunits are found in CP (β1, β2, β3) and these receptors exist in all vertebrate organisms. In previous work, we have shown that the β1 and β2 subunits have distinct functions in cardiac myocytes that cannot be carried out by the other isoform. Furthermore, our data suggests that the unique function of the β isoforms Involves their interactions with an additional protein. We are using a genetic screen, a yeast two-hybrid screen, to identify proteins that interact with each β subunit.