Identification of Interacting Proteins
Location
CSU
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Marilyn Hart
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
Capping protein (CP) is an actin binding protein that associates with the actin cytoskeleton and regulates its assembly. CP, found in all eucaryotic cells, is a heterodimer composed of α and β subunits. Three β subunits are found in CP (β1, β2, β3) and these receptors exist in all vertebrate organisms. In previous work, we have shown that the β1 and β2 subunits have distinct functions in cardiac myocytes that cannot be carried out by the other isoform. Furthermore, our data suggests that the unique function of the β isoforms Involves their interactions with an additional protein. We are using a genetic screen, a yeast two-hybrid screen, to identify proteins that interact with each β subunit.
Identification of Interacting Proteins
CSU
Capping protein (CP) is an actin binding protein that associates with the actin cytoskeleton and regulates its assembly. CP, found in all eucaryotic cells, is a heterodimer composed of α and β subunits. Three β subunits are found in CP (β1, β2, β3) and these receptors exist in all vertebrate organisms. In previous work, we have shown that the β1 and β2 subunits have distinct functions in cardiac myocytes that cannot be carried out by the other isoform. Furthermore, our data suggests that the unique function of the β isoforms Involves their interactions with an additional protein. We are using a genetic screen, a yeast two-hybrid screen, to identify proteins that interact with each β subunit.
