Quantification of Malondialdehyde in Exercise Participants

Location

CSU 253

Start Date

26-4-2005 10:00 AM

End Date

26-4-2005 12:00 PM

Student's Major

Chemistry and Geology

Student's College

Science, Engineering and Technology

Mentor's Name

Mary Hadley

Mentor's Department

Chemistry and Geology

Mentor's College

Science, Engineering and Technology

Second Mentor's Name

Mary Visser

Second Mentor's Department

Human Performance

Second Mentor's College

Allied Health and Nursing

Description

Malondialdehyde (MDA) is a byproduct of lipid peroxidation (LP) and its production in vivo should increase with exercise. Increased LP has been correlated with increased incidences of adverse physiological conditions such as heart disease. It may be beneficial for those who exercise regularly to increase their intake of the antioxidant vitamin E to decrease LP. This pilot experiment was conducted to determine if supplementation of vitamin E would decrease urinary MDA excretion in non-athletes who participate in a prescribed exercise regiment (IRB log 1973 ). Urine and dietary diet records were collected from the subjects, some received a daily vitamin E supplement. The urine was treated with thiobarbituric acid (TEA) and the resulting TBA-MDA complex was quantified using a high pressure liquid chromatography system. Although the number of subjects volunteering for this experiment was too small for any conclusions to be drawn, it appears that the subjects who received the vitamin E had reduced levels of MDA after 8 weeks of supplementation. To reach any conclusion the experiment must be repeated with more subjects.

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Apr 26th, 10:00 AM Apr 26th, 12:00 PM

Quantification of Malondialdehyde in Exercise Participants

CSU 253

Malondialdehyde (MDA) is a byproduct of lipid peroxidation (LP) and its production in vivo should increase with exercise. Increased LP has been correlated with increased incidences of adverse physiological conditions such as heart disease. It may be beneficial for those who exercise regularly to increase their intake of the antioxidant vitamin E to decrease LP. This pilot experiment was conducted to determine if supplementation of vitamin E would decrease urinary MDA excretion in non-athletes who participate in a prescribed exercise regiment (IRB log 1973 ). Urine and dietary diet records were collected from the subjects, some received a daily vitamin E supplement. The urine was treated with thiobarbituric acid (TEA) and the resulting TBA-MDA complex was quantified using a high pressure liquid chromatography system. Although the number of subjects volunteering for this experiment was too small for any conclusions to be drawn, it appears that the subjects who received the vitamin E had reduced levels of MDA after 8 weeks of supplementation. To reach any conclusion the experiment must be repeated with more subjects.