Polymer Brushes as Potential Supports for Protein Microarrays
Location
CSU 253
Start Date
26-4-2005 10:00 AM
End Date
26-4-2005 12:00 PM
Student's Major
Chemistry and Geology
Student's College
Science, Engineering and Technology
Mentor's Name
Merlin L. Bruening, Dept. of Chemistry, Michigan State University
Second Mentor's Name
Matthew D. Miller, Dept. of Chemistry, Michigan State University
Description
Protein microarrays can be used to track protein interactions with DNA, lipids, antibodies, and other proteins. Applications include functional and pathway determinations and disease screening. This research aims at controlling the density of poly(2-hydroxylethyl methacrylate) (PHEMA) brushes brushes to tailor these surfaces for optimal attachment (amount and activity) of protein microarrays. The terminal hydroxyl group of PHEMA was derivatized to immobilize proteins on the polymer brushes through amide bond formation. Derivatization of PHEMA was accomplished by reaction with succinic anhydride followed by activation with N-hydroxysuccinimide and this process was monitored by reflectance FTIR spectroscopy and ellipsometry. A fluorescent antibody was attached to an immobilized protein, and the protein-antibody interaction was monitored with a microarray scanner. Low-density PHEMA brushes showed a higher immobilization than denser films.
Polymer Brushes as Potential Supports for Protein Microarrays
CSU 253
Protein microarrays can be used to track protein interactions with DNA, lipids, antibodies, and other proteins. Applications include functional and pathway determinations and disease screening. This research aims at controlling the density of poly(2-hydroxylethyl methacrylate) (PHEMA) brushes brushes to tailor these surfaces for optimal attachment (amount and activity) of protein microarrays. The terminal hydroxyl group of PHEMA was derivatized to immobilize proteins on the polymer brushes through amide bond formation. Derivatization of PHEMA was accomplished by reaction with succinic anhydride followed by activation with N-hydroxysuccinimide and this process was monitored by reflectance FTIR spectroscopy and ellipsometry. A fluorescent antibody was attached to an immobilized protein, and the protein-antibody interaction was monitored with a microarray scanner. Low-density PHEMA brushes showed a higher immobilization than denser films.
