Polymer Brushes as Potential Supports for Protein Microarrays

Location

CSU 253

Start Date

26-4-2005 10:00 AM

End Date

26-4-2005 12:00 PM

Student's Major

Chemistry and Geology

Student's College

Science, Engineering and Technology

Mentor's Name

Merlin L. Bruening, Dept. of Chemistry, Michigan State University

Second Mentor's Name

Matthew D. Miller, Dept. of Chemistry, Michigan State University

Description

Protein microarrays can be used to track protein interactions with DNA, lipids, antibodies, and other proteins. Applications include functional and pathway determinations and disease screening. This research aims at controlling the density of poly(2-hydroxylethyl methacrylate) (PHEMA) brushes brushes to tailor these surfaces for optimal attachment (amount and activity) of protein microarrays. The terminal hydroxyl group of PHEMA was derivatized to immobilize proteins on the polymer brushes through amide bond formation. Derivatization of PHEMA was accomplished by reaction with succinic anhydride followed by activation with N-hydroxysuccinimide and this process was monitored by reflectance FTIR spectroscopy and ellipsometry. A fluorescent antibody was attached to an immobilized protein, and the protein-antibody interaction was monitored with a microarray scanner. Low-density PHEMA brushes showed a higher immobilization than denser films.

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Apr 26th, 10:00 AM Apr 26th, 12:00 PM

Polymer Brushes as Potential Supports for Protein Microarrays

CSU 253

Protein microarrays can be used to track protein interactions with DNA, lipids, antibodies, and other proteins. Applications include functional and pathway determinations and disease screening. This research aims at controlling the density of poly(2-hydroxylethyl methacrylate) (PHEMA) brushes brushes to tailor these surfaces for optimal attachment (amount and activity) of protein microarrays. The terminal hydroxyl group of PHEMA was derivatized to immobilize proteins on the polymer brushes through amide bond formation. Derivatization of PHEMA was accomplished by reaction with succinic anhydride followed by activation with N-hydroxysuccinimide and this process was monitored by reflectance FTIR spectroscopy and ellipsometry. A fluorescent antibody was attached to an immobilized protein, and the protein-antibody interaction was monitored with a microarray scanner. Low-density PHEMA brushes showed a higher immobilization than denser films.