Initial Comparison of DNA Sequence Variation Between a 5-SNP Cluster on the Y-Chromosome and an SNP-Rich Region of the Mitochondrial DNA
Location
CSU 253
Start Date
24-4-2006 10:30 AM
End Date
24-4-2006 12:00 PM
Student's Major
Chemistry and Geology
Student's College
Science, Engineering and Technology
Mentor's Name
James Rife
Mentor's Department
Chemistry and Geology
Mentor's College
Science, Engineering and Technology
Second Mentor's Name
Theresa Salerno
Second Mentor's Department
Chemistry and Geology
Second Mentor's College
Science, Engineering and Technology
Description
Mitochondrial DNA (mtDNA) and Y-chromosomal DNA, inherited from the mother and father respectively, are extensively used for lineage studies(Underhill, P. A. et al.. Nature Genetics, 26,pp. 358-361, Nov. 2000). Comparisons of the geographical regions of origin and the presence of certain SNPs (single nucleotide polymorphisms) on both the Y-chromosomal DNA and the mtDNA have provided useful information in the fields of forensic work and anthropology. However, the Y Chromosome only reflects a single lineage for an individual rather than the individual's very complex heredity. Use of mtDNA is similarly limited. The goal of this project is to investigate correlations in diversity between these two sources of DNA to see if they reflect similar geographical heredity. In this research project, DNA samples were collected from males with diverse geographical backgrounds, amplified by the Polymerase Chain Reaction (PCR) and sequenced to determine if any SNP variations were present. The primers were designed to allow PCR amplification of a cluster supposedly containing five SNPs between positions 20,758,511 and 20,758,579 on the Ychromosome. The same samples were also amplified using primers that amplify an SNP-rich region of mtDNA between positions 4,200 and 4,600. Observed SNPs were matched with published frequencies in various populations. Correlations were made between the likely geographic origin indicated by the Y-SNPs and the mt-SNPs and the actual ethnic origin of the donor.
Initial Comparison of DNA Sequence Variation Between a 5-SNP Cluster on the Y-Chromosome and an SNP-Rich Region of the Mitochondrial DNA
CSU 253
Mitochondrial DNA (mtDNA) and Y-chromosomal DNA, inherited from the mother and father respectively, are extensively used for lineage studies(Underhill, P. A. et al.. Nature Genetics, 26,pp. 358-361, Nov. 2000). Comparisons of the geographical regions of origin and the presence of certain SNPs (single nucleotide polymorphisms) on both the Y-chromosomal DNA and the mtDNA have provided useful information in the fields of forensic work and anthropology. However, the Y Chromosome only reflects a single lineage for an individual rather than the individual's very complex heredity. Use of mtDNA is similarly limited. The goal of this project is to investigate correlations in diversity between these two sources of DNA to see if they reflect similar geographical heredity. In this research project, DNA samples were collected from males with diverse geographical backgrounds, amplified by the Polymerase Chain Reaction (PCR) and sequenced to determine if any SNP variations were present. The primers were designed to allow PCR amplification of a cluster supposedly containing five SNPs between positions 20,758,511 and 20,758,579 on the Ychromosome. The same samples were also amplified using primers that amplify an SNP-rich region of mtDNA between positions 4,200 and 4,600. Observed SNPs were matched with published frequencies in various populations. Correlations were made between the likely geographic origin indicated by the Y-SNPs and the mt-SNPs and the actual ethnic origin of the donor.
Recommended Citation
Burns, Michael Bradley and Cassidy Punt. "Initial Comparison of DNA Sequence Variation Between a 5-SNP Cluster on the Y-Chromosome and an SNP-Rich Region of the Mitochondrial DNA." Undergraduate Research Symposium, Mankato, MN, April 24, 2006.
https://cornerstone.lib.mnsu.edu/urs/2006/oral-session-D/4
