Initial Comparison of DNA Sequence Variation Between a 5-SNP Cluster on the Y-Chromosome and an SNP-Rich Region of the Mitochondrial DNA

Location

CSU 253

Start Date

24-4-2006 10:30 AM

End Date

24-4-2006 12:00 PM

Student's Major

Chemistry and Geology

Student's College

Science, Engineering and Technology

Mentor's Name

James Rife

Mentor's Department

Chemistry and Geology

Mentor's College

Science, Engineering and Technology

Second Mentor's Name

Theresa Salerno

Second Mentor's Department

Chemistry and Geology

Second Mentor's College

Science, Engineering and Technology

Description

Mitochondrial DNA (mtDNA) and Y-chromosomal DNA, inherited from the mother and father respectively, are extensively used for lineage studies(Underhill, P. A. et al.. Nature Genetics, 26,pp. 358-361, Nov. 2000). Comparisons of the geographical regions of origin and the presence of certain SNPs (single nucleotide polymorphisms) on both the Y-chromosomal DNA and the mtDNA have provided useful information in the fields of forensic work and anthropology. However, the Y Chromosome only reflects a single lineage for an individual rather than the individual's very complex heredity. Use of mtDNA is similarly limited. The goal of this project is to investigate correlations in diversity between these two sources of DNA to see if they reflect similar geographical heredity. In this research project, DNA samples were collected from males with diverse geographical backgrounds, amplified by the Polymerase Chain Reaction (PCR) and sequenced to determine if any SNP variations were present. The primers were designed to allow PCR amplification of a cluster supposedly containing five SNPs between positions 20,758,511 and 20,758,579 on the Ychromosome. The same samples were also amplified using primers that amplify an SNP-rich region of mtDNA between positions 4,200 and 4,600. Observed SNPs were matched with published frequencies in various populations. Correlations were made between the likely geographic origin indicated by the Y-SNPs and the mt-SNPs and the actual ethnic origin of the donor.

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Apr 24th, 10:30 AM Apr 24th, 12:00 PM

Initial Comparison of DNA Sequence Variation Between a 5-SNP Cluster on the Y-Chromosome and an SNP-Rich Region of the Mitochondrial DNA

CSU 253

Mitochondrial DNA (mtDNA) and Y-chromosomal DNA, inherited from the mother and father respectively, are extensively used for lineage studies(Underhill, P. A. et al.. Nature Genetics, 26,pp. 358-361, Nov. 2000). Comparisons of the geographical regions of origin and the presence of certain SNPs (single nucleotide polymorphisms) on both the Y-chromosomal DNA and the mtDNA have provided useful information in the fields of forensic work and anthropology. However, the Y Chromosome only reflects a single lineage for an individual rather than the individual's very complex heredity. Use of mtDNA is similarly limited. The goal of this project is to investigate correlations in diversity between these two sources of DNA to see if they reflect similar geographical heredity. In this research project, DNA samples were collected from males with diverse geographical backgrounds, amplified by the Polymerase Chain Reaction (PCR) and sequenced to determine if any SNP variations were present. The primers were designed to allow PCR amplification of a cluster supposedly containing five SNPs between positions 20,758,511 and 20,758,579 on the Ychromosome. The same samples were also amplified using primers that amplify an SNP-rich region of mtDNA between positions 4,200 and 4,600. Observed SNPs were matched with published frequencies in various populations. Correlations were made between the likely geographic origin indicated by the Y-SNPs and the mt-SNPs and the actual ethnic origin of the donor.

Recommended Citation

Burns, Michael Bradley and Cassidy Punt. "Initial Comparison of DNA Sequence Variation Between a 5-SNP Cluster on the Y-Chromosome and an SNP-Rich Region of the Mitochondrial DNA." Undergraduate Research Symposium, Mankato, MN, April 24, 2006.
https://cornerstone.lib.mnsu.edu/urs/2006/oral-session-D/4