Use of Mycobacterium Smegmatis to Express a Recombinant Mycobacterial Protein
Location
CSU North Ballroom
Start Date
25-4-2006 10:00 AM
End Date
25-4-2006 12:00 PM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
Timothy Secott
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
Among the major goals of vaccine development are to produce products that are inexpensive, effective, and have few side effects. Protein products of pathogenic microorganisms may afford such opportunities, as subunit vaccines. It is often possible to express proteins of interest with catalog Escherichia coli strains. However, this process can be ineffective if the protein of interest comes from organisms such as Mycobacterium spp. that have a genome composition that is significantly different from that of E.coli. The purpose of this study was to attempt to use Mycobacterium smegmatis a non-pathogenic organism, to express a mycobacterial protein. We have cloned a histidine tagged mce gene (a potential virulence factor expressed by Mycobacterium paratuberculosis) into a mycobacterial shuttle plasmid. Protein gel electrophoresis and western blotting will used to compare the expression of mce in M. smegmatis with that from a similar plasmid construct introduce into E.coli.
Use of Mycobacterium Smegmatis to Express a Recombinant Mycobacterial Protein
CSU North Ballroom
Among the major goals of vaccine development are to produce products that are inexpensive, effective, and have few side effects. Protein products of pathogenic microorganisms may afford such opportunities, as subunit vaccines. It is often possible to express proteins of interest with catalog Escherichia coli strains. However, this process can be ineffective if the protein of interest comes from organisms such as Mycobacterium spp. that have a genome composition that is significantly different from that of E.coli. The purpose of this study was to attempt to use Mycobacterium smegmatis a non-pathogenic organism, to express a mycobacterial protein. We have cloned a histidine tagged mce gene (a potential virulence factor expressed by Mycobacterium paratuberculosis) into a mycobacterial shuttle plasmid. Protein gel electrophoresis and western blotting will used to compare the expression of mce in M. smegmatis with that from a similar plasmid construct introduce into E.coli.
Recommended Citation
Johnson, Robert. "Use of Mycobacterium Smegmatis to Express a Recombinant Mycobacterial Protein." Undergraduate Research Symposium, Mankato, MN, April 25, 2006.
https://cornerstone.lib.mnsu.edu/urs/2006/poster-session-D/8
