Use of Mycobacterium Smegmatis to Express a Recombinant Mycobacterial Protein

Location

CSU North Ballroom

Start Date

25-4-2006 10:00 AM

End Date

25-4-2006 12:00 PM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Timothy Secott

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

Among the major goals of vaccine development are to produce products that are inexpensive, effective, and have few side effects. Protein products of pathogenic microorganisms may afford such opportunities, as subunit vaccines. It is often possible to express proteins of interest with catalog Escherichia coli strains. However, this process can be ineffective if the protein of interest comes from organisms such as Mycobacterium spp. that have a genome composition that is significantly different from that of E.coli. The purpose of this study was to attempt to use Mycobacterium smegmatis a non-pathogenic organism, to express a mycobacterial protein. We have cloned a histidine tagged mce gene (a potential virulence factor expressed by Mycobacterium paratuberculosis) into a mycobacterial shuttle plasmid. Protein gel electrophoresis and western blotting will used to compare the expression of mce in M. smegmatis with that from a similar plasmid construct introduce into E.coli.

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Apr 25th, 10:00 AM Apr 25th, 12:00 PM

Use of Mycobacterium Smegmatis to Express a Recombinant Mycobacterial Protein

CSU North Ballroom

Among the major goals of vaccine development are to produce products that are inexpensive, effective, and have few side effects. Protein products of pathogenic microorganisms may afford such opportunities, as subunit vaccines. It is often possible to express proteins of interest with catalog Escherichia coli strains. However, this process can be ineffective if the protein of interest comes from organisms such as Mycobacterium spp. that have a genome composition that is significantly different from that of E.coli. The purpose of this study was to attempt to use Mycobacterium smegmatis a non-pathogenic organism, to express a mycobacterial protein. We have cloned a histidine tagged mce gene (a potential virulence factor expressed by Mycobacterium paratuberculosis) into a mycobacterial shuttle plasmid. Protein gel electrophoresis and western blotting will used to compare the expression of mce in M. smegmatis with that from a similar plasmid construct introduce into E.coli.

Recommended Citation

Johnson, Robert. "Use of Mycobacterium Smegmatis to Express a Recombinant Mycobacterial Protein." Undergraduate Research Symposium, Mankato, MN, April 25, 2006.
https://cornerstone.lib.mnsu.edu/urs/2006/poster-session-D/8