Examination of Proteins in Roundup Ready Soybean Seeds
Location
CSU North Ballroom
Start Date
25-4-2006 10:00 AM
End Date
25-4-2006 12:00 PM
Student's Major
Chemistry and Geology
Student's College
Science, Engineering and Technology
Mentor's Name
Theresa Salerno
Mentor's Department
Chemistry and Geology
Mentor's College
Science, Engineering and Technology
Description
Glyphosate is a non-selective herbicide used for weed control. The presence of glyphosate inhibits the shikimate pathway enzyme, 5-enolpyruvylshikimate 3-phosphate (EPS?) synthase, thereby preventing biosynthesis of aromatic amino acids that are required for normal plant growth. Roundup ready soybean plants have been genetically modified to make a different kind of EPS? synthase, one that is not inhibited by glyphosate. Several proteins exist in soybean seeds. By optimizing extraction conditions, several of these proteins can be analyzed by two dimensional (2D) electrophoresis which separates first by charge and second by size. This study tested the hypothesis that introduction of a new EPSP synthase might alter soybean seed protein 2-D electrophoresis patterns. Two dimensional patterns of control versus Roundup ready soybean seeds will be compared.
Examination of Proteins in Roundup Ready Soybean Seeds
CSU North Ballroom
Glyphosate is a non-selective herbicide used for weed control. The presence of glyphosate inhibits the shikimate pathway enzyme, 5-enolpyruvylshikimate 3-phosphate (EPS?) synthase, thereby preventing biosynthesis of aromatic amino acids that are required for normal plant growth. Roundup ready soybean plants have been genetically modified to make a different kind of EPS? synthase, one that is not inhibited by glyphosate. Several proteins exist in soybean seeds. By optimizing extraction conditions, several of these proteins can be analyzed by two dimensional (2D) electrophoresis which separates first by charge and second by size. This study tested the hypothesis that introduction of a new EPSP synthase might alter soybean seed protein 2-D electrophoresis patterns. Two dimensional patterns of control versus Roundup ready soybean seeds will be compared.
Recommended Citation
Denge, Fiona. "Examination of Proteins in Roundup Ready Soybean Seeds." Undergraduate Research Symposium, Mankato, MN, April 25, 2006.
https://cornerstone.lib.mnsu.edu/urs/2006/poster-session-E/1
