Event Title

Examination of Proteins in Roundup Ready Soybean Seeds

Location

CSU North Ballroom

Start Date

25-4-2006 10:00 AM

End Date

25-4-2006 12:00 PM

Student's Major

Chemistry and Geology

Student's College

Science, Engineering and Technology

Mentor's Name

Theresa Salerno

Mentor's Department

Chemistry and Geology

Mentor's College

Science, Engineering and Technology

Description

Glyphosate is a non-selective herbicide used for weed control. The presence of glyphosate inhibits the shikimate pathway enzyme, 5-enolpyruvylshikimate 3-phosphate (EPS?) synthase, thereby preventing biosynthesis of aromatic amino acids that are required for normal plant growth. Roundup ready soybean plants have been genetically modified to make a different kind of EPS? synthase, one that is not inhibited by glyphosate. Several proteins exist in soybean seeds. By optimizing extraction conditions, several of these proteins can be analyzed by two dimensional (2D) electrophoresis which separates first by charge and second by size. This study tested the hypothesis that introduction of a new EPSP synthase might alter soybean seed protein 2-D electrophoresis patterns. Two dimensional patterns of control versus Roundup ready soybean seeds will be compared.

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Apr 25th, 10:00 AM Apr 25th, 12:00 PM

Examination of Proteins in Roundup Ready Soybean Seeds

CSU North Ballroom

Glyphosate is a non-selective herbicide used for weed control. The presence of glyphosate inhibits the shikimate pathway enzyme, 5-enolpyruvylshikimate 3-phosphate (EPS?) synthase, thereby preventing biosynthesis of aromatic amino acids that are required for normal plant growth. Roundup ready soybean plants have been genetically modified to make a different kind of EPS? synthase, one that is not inhibited by glyphosate. Several proteins exist in soybean seeds. By optimizing extraction conditions, several of these proteins can be analyzed by two dimensional (2D) electrophoresis which separates first by charge and second by size. This study tested the hypothesis that introduction of a new EPSP synthase might alter soybean seed protein 2-D electrophoresis patterns. Two dimensional patterns of control versus Roundup ready soybean seeds will be compared.

Recommended Citation

Denge, Fiona. "Examination of Proteins in Roundup Ready Soybean Seeds." Undergraduate Research Symposium, Mankato, MN, April 25, 2006.
https://cornerstone.lib.mnsu.edu/urs/2006/poster-session-E/1