Colocalization of FAM171B, CAMKIID, and MPP3 Using Co-Immunofluorescence

Location

CSU Ballroom

Start Date

2-4-2019 10:00 AM

End Date

2-4-2019 11:30 AM

Student's Major

Biological Sciences

Student's College

Science, Engineering and Technology

Mentor's Name

Geoffrey Goellner

Mentor's Department

Biological Sciences

Mentor's College

Science, Engineering and Technology

Description

Neurodegenerative diseases, such as Huntington's diseases (HD), are linked to polyglutamine (PolyQ) disorders. The continuous repetition of the glutamine amino acid is linked to these neurodegenerative and genetic disorders. The expanded sequence of Glutamine is found in affected individuals and thought to be the underlying cause of neurodegenerative diseases such as HD. Poly length variations are found in all individuals but those exceeding the disease's threshold develop the disease. Previous studies have identified various proteins that contain these PolyQ stretches but their functions have yet to be determined. One of those PolyQ proteins is FAM171B, a relatively new discovered protein that has shown high expressivity in neurons. Previous studies have also suggested a colocalization between proteins CAMKIID along with MPP3. Using precise technique and concrete procedure, a discovery in the colocalization between FAM171B, CAMKIID and MPP3 is very much possible. Using Co-Immunofluorescence, it is possible to determine whether the colocalization is present between these proteins. Using red and green fluorophores, as markers for these proteins, if there a yellow signal is detected, we can presume colocalization is occurring. Preliminary data supports that FAM171B is perinuclear as well as throughout the cytoplasm. Early microscopy data suggests that CAMKIID and MMP3 are thought to be located all throughout the cytoplasm with no particular location. Different fixative will be used to determine which is better for future experiments.

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Apr 2nd, 10:00 AM Apr 2nd, 11:30 AM

Colocalization of FAM171B, CAMKIID, and MPP3 Using Co-Immunofluorescence

CSU Ballroom

Neurodegenerative diseases, such as Huntington's diseases (HD), are linked to polyglutamine (PolyQ) disorders. The continuous repetition of the glutamine amino acid is linked to these neurodegenerative and genetic disorders. The expanded sequence of Glutamine is found in affected individuals and thought to be the underlying cause of neurodegenerative diseases such as HD. Poly length variations are found in all individuals but those exceeding the disease's threshold develop the disease. Previous studies have identified various proteins that contain these PolyQ stretches but their functions have yet to be determined. One of those PolyQ proteins is FAM171B, a relatively new discovered protein that has shown high expressivity in neurons. Previous studies have also suggested a colocalization between proteins CAMKIID along with MPP3. Using precise technique and concrete procedure, a discovery in the colocalization between FAM171B, CAMKIID and MPP3 is very much possible. Using Co-Immunofluorescence, it is possible to determine whether the colocalization is present between these proteins. Using red and green fluorophores, as markers for these proteins, if there a yellow signal is detected, we can presume colocalization is occurring. Preliminary data supports that FAM171B is perinuclear as well as throughout the cytoplasm. Early microscopy data suggests that CAMKIID and MMP3 are thought to be located all throughout the cytoplasm with no particular location. Different fixative will be used to determine which is better for future experiments.

Recommended Citation

Ekowa, Dilibe. "Colocalization of FAM171B, CAMKIID, and MPP3 Using Co-Immunofluorescence." Undergraduate Research Symposium, Mankato, MN, April 2, 2019.
https://cornerstone.lib.mnsu.edu/urs/2019/poster-session-A/19