Characterization of Insulin-Like Growth Factor 1 (Igf1) Protein Levels in a Mouse Model that over Expresses Igf1
Location
CSU Ballroom
Start Date
2-4-2019 10:00 AM
End Date
2-4-2019 11:30 AM
Student's Major
Biological Sciences
Student's College
Science, Engineering and Technology
Mentor's Name
David Sharlin
Mentor's Department
Biological Sciences
Mentor's College
Science, Engineering and Technology
Description
Congenital hypothyroidism is a condition in which there is an inadequate amount of circulating thyroid hormone during development and can lead to many adverse effects, including permanent neurological deficits. Interestingly, developmental inefficiency of brain-derived Insulin-Like Growth Factor 1 (Igf1) results in neurological defects that are similar to those associated with developmental hypothyroidism. Considering this, we are investigating whether ectopic expression of Igf1 can restore normal brain development in the face of low thyroid hormone.
To test this, we are utilizing the “Tet-Off” system in mice to overexpress Igf1 specifically in the brain. In this system, double transgenic mice are generated. One transgene has an astrocyte-specific promoter and codes for the tetracycline transactivator (tTA). The other transgene contains the tetracycline response element (TRE), which drives Igf1 production when bound to the tTA. Two groups of wildtype and double transgenic mice will be utilized: control and hypothyroid. The mice will be sacrificed on postnatal day 15, at which time the cerebral cortex and hippocampus will be dissected out. Enzyme-linked immunosorbent assay (ELISA) will be used to determine Igf1 levels in the cortex and hippocampus.
We predict that Igf1 proteins levels will be significantly increased in euthyroid double transgenic brains compared to hypothyroid double transgenics. Additionally, we predict that hypothyroid double transgenic mice will have similar or increased Igf1 levels compared to euthyroid wildtypes. These findings would validate our model and allow us to ask whether restoring brain Igf1 levels in hypothyroidism can rescue developmental effects associated with low thyroid hormone in development.
Characterization of Insulin-Like Growth Factor 1 (Igf1) Protein Levels in a Mouse Model that over Expresses Igf1
CSU Ballroom
Congenital hypothyroidism is a condition in which there is an inadequate amount of circulating thyroid hormone during development and can lead to many adverse effects, including permanent neurological deficits. Interestingly, developmental inefficiency of brain-derived Insulin-Like Growth Factor 1 (Igf1) results in neurological defects that are similar to those associated with developmental hypothyroidism. Considering this, we are investigating whether ectopic expression of Igf1 can restore normal brain development in the face of low thyroid hormone.
To test this, we are utilizing the “Tet-Off” system in mice to overexpress Igf1 specifically in the brain. In this system, double transgenic mice are generated. One transgene has an astrocyte-specific promoter and codes for the tetracycline transactivator (tTA). The other transgene contains the tetracycline response element (TRE), which drives Igf1 production when bound to the tTA. Two groups of wildtype and double transgenic mice will be utilized: control and hypothyroid. The mice will be sacrificed on postnatal day 15, at which time the cerebral cortex and hippocampus will be dissected out. Enzyme-linked immunosorbent assay (ELISA) will be used to determine Igf1 levels in the cortex and hippocampus.
We predict that Igf1 proteins levels will be significantly increased in euthyroid double transgenic brains compared to hypothyroid double transgenics. Additionally, we predict that hypothyroid double transgenic mice will have similar or increased Igf1 levels compared to euthyroid wildtypes. These findings would validate our model and allow us to ask whether restoring brain Igf1 levels in hypothyroidism can rescue developmental effects associated with low thyroid hormone in development.
Recommended Citation
Ouadipe, Anjola. "Characterization of Insulin-Like Growth Factor 1 (Igf1) Protein Levels in a Mouse Model that over Expresses Igf1." Undergraduate Research Symposium, Mankato, MN, April 2, 2019.
https://cornerstone.lib.mnsu.edu/urs/2019/poster-session-A/22
